Inflammation and Cancer

In this book, expert researchers deliver a systematic guide to techniques addressing various aspects of experimental cancer biology. The book selectively focuses on inflammation-mediated tumorigenesis, while promoting improvisations on a per-case basis.

According to the most recent clinical oncology data, one out of seven newly diagnosed malignancies worldwide result from infection and chronic inflammation in conjunction with cancer. In Inflammation and Cancer: Methods and Protocols, expert researchers deliver a systematic guide to techniques addressing various aspects of experimental cancer biology, selectively focused on inflammation-mediated tumorigenesis, while promoting improvisations on a per-case basis. Volume 1, Experimental Models and Practical Approaches provides an overview of a spectrum of techniques developed to analyze the outcomes of inflammation-mediated carcinogenesis on the tissue, cellular, and molecular levels while highlighting several diagnostic aspects, such as biomarker discovery and molecular signatures evaluation. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step laboratory protocols, and a notes section, which examines tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Inflammation and Cancer: Methods and Protocols promises to serve as a vital guide and resource for investigators and clinicians working toward the goal of combatting the estimated 2, 200 inflammation-related oncogenesis fatalities occurring every day.

Contains key readily reproducible experimental approaches certain to be useful as technologies continue to develop Details basic requirements for the most recent techniques still awaiting experimental substantiation Fosters rigorous scientific interactions among basic and clinical researchers aimed at further molecular demarcation of the elaborate pathways leading from inflammation to tumor formation Compiles contemporary laboratory techniques with an emphasis on carcinogenic events mediated by chronic inflammation and pathogen infection Includes supplementary material: sn.pub/extras

Inhalt
Experimental Approaches to Study Chronic Inflammation Related Carcinogenesis.- Collection and Preparation of Rodent Tissue Samples for Histopathological and Molecular Studies in Carcinogenesis.- Biomarkers of Cell Proliferation in Carcinomas: Detection of Angiogenesis and Infiltrated Leukocytes.- Assessment of Local Proteolytic Milieu as a Factor in Tumor Invasiveness and Metastasis Formation: In Vitro Collagen Degradation and Invasion Assays.- Cytokine Multiplex Analysis.- Approaches to Determine Expression of Inflammatory Cytokines.- Selective Immortalization of Tumor-Specific T Cells to Establish Long-Term T-Cell Lines Maintaining Primary Cell Characteristics.- YKL-40: A Novel Marker Shared by Chronic Inflammation and Oncogenic Transformation.- Angiogenesis Links Chronic Inflammation with Cancer.- Inflammatory Bowel Disease: A Model of Chronic Inflammation-Induced Cancer.- Oncogenic Potential of Inflammation Induced by Viral and Bacterial Infections.- Gastric Carcinogenesis and Helicobacter pylori Infection.- Helicobacter -Based Mouse Models of Digestive System Carcinogenesis.- Screening for Molecular Markers of Cervical Papillomavirus Infection: Overview of Methods and Their Clinical Implications.- Screening for EpsteinBarr Virus in Hodgkin's Lymphoma.- A Hepatitis C Virus Xenograft Mouse Efficacy Model.- Gene Expression Profiling in Cervical Cancer: Identification of Novel Markers for Disease Diagnosis and Therapy.- Detection and Genotyping Analysis of Human Papillomavirus Isolates from Liquid-Based Cervical Cytology Specimens.