Integrated Methods in Protein Biochemistry: Part A: Volume 675

Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more.

Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more.

Autorentext
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla's research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Inhalt

1. DNA and protein engineering to create protein bioswitches with new functions
   Stewart Loh
2. Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases
   Susan Michaelis
3. An experimental protocol to study lipid transfer proteins
   Haijia Yu
4. Synthesis of small heat shock proteins
   Matthew Pratt
5. Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins
   Guang-Yu Yang
6. An experimental protocol for glycoconjugate analysis
   Yifa Zhou
7. Methods for proximity-based biotinylation combined with Mass Spectrometry
   Ralph Kehlenbach
8. Synthetic antibody fragments as conformational sensors of protein activation and trafficking
   Arun K. Shukla
9. Expression, purification, functional analysis and crystallization of Rag GTPase
   Kuang Shen
10. Purification of bacterial transcription elongation complexes by photoreversible immobilization
    Eric Strobel
11. Inhibition of c-Myc-MAX heterodimerization
    Neha Garg
12. Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases
    Markus C. Wahl
13. Phosphorylation and subcellular localization of human phospholipase A1
    Atsushi Yamashita
14. Integrated methods in studying light-sensitive proteins
    Lars-Oliver Essen
15. Structure and biochemistry of thermophilic protein
    Ankan Banerjee
16. Using hydrogen/deuterium exchange mass spectrometry to probe protein structure
    Gert Bange
17. Nanopore force spectroscopy to probe structural dynamics of proteins
    Sonja Schmid
18. Structural analysis of knotted proteins
    Shang-Te "Danny" Hsu
19. Mutation-induced structural changes in the SARC-CoV2 spike protein
    Shang-Te "Danny" Hsu
20. Preparing metabolite capped RNA substrates using RNA polymerases
    Jeremy G. Bird