Intermediate Filament Associated Proteins: Volume 569

Intermediate Filament Associated Proteins, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers.

Autorentext
Department of Cell Biology, The Johns Hopkins University School of Medicine, USA Department of Cell Biology, The Netherlands Cancer Institute, The Netherlands

Zusammenfassung
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Inhalt
Part I: Identification and Biochemical Analysis of Lamin-Associated Proteins

1. BioID Identification of Lamin-Associated Proteins
   Aaron A. Mehus, Ruthellen H. Anderson and Kyle J. Roux
2. Proximity-Dependent Biotin Identification (BioID) in Dictyostelium Amoebae
   Petros Batsios, Irene Meyer and Ralph Gräf
3. Purification and Structural Analysis of LEM-Domain Proteins
   Isaline Herrada, Benjamin Bourgeois, Camille Samson, Brigitte Buendia, Howard J Worman and Sophie Zinn-Justin
4. Purification and Structural Analysis of SUN and KASH Domain Proteins
   F. Esra Demircioglu, Victor E. Cruz and Thomas U. Schwartz
5. Purification of Lamins and Soluble Fragments of NETs
   Alexandr A. Makarov, Andrea Rizzotto, Peter Meinke and Eric C. Schirmer
6. Simple Separation of Functionally-Distinct Populations of Lamin-Binding Proteins
   Jason M. Berk and Katherine L. Wilson
   Part II: General Methods for Analysis of Intermediate Filament-Associated Proteins
7. Development of a Novel Green Fluorescent Protein-Based Binding Assay to Study the Association of Plakins with Intermediate Filament Proteins
   Bertrand Favre, Nadja Begré, Jamal-Eddine Bouameur, and Luca Borradori
8. Functional Analysis of Keratin-Associated Proteins in Intestinal Epithelia: Heat- Shock Protein Chaperoning and Kinase Rescue
   Anastasia Mashukova, Radia Forteza and Pedro J. Salas
9. Purification of Protein Chaperones and their Functional Assays with Intermediate Filaments
   Ming Der Perng, Yu-Shan Huang and Roy A. Quinlan
10. Purification and Structural Analysis of Plectin and BPAG1e
    José A Manso, Inés García Rubio, María Gómez-Hernández, Esther Ortega, Rubén M Buey, Ana M Carballido, Arturo Carabias, Noelia Alonso-García and José M de Pereda
11. Purification and Structural Analysis of Desmoplakin
    Hee-Jung Choi and William I. Weis
12. Degradation of the Intermediate Filament Family by Gigaxonin
    Pascale Bomont
    Part III: Functional and Genetic Analysis of the Plakin and Other Cytoskeletal Cross-Linkers
13. Functional and Genetic Analysis of Plectin in Skin And Muscle
    Günther A. Rezniczek, Lilli Winter, Gernot Walko, and Gerhard Wiche
14. Functional and Genetic Analysis of Epiplakin in Epithelial Cells
    Sandra Szabo, Karl L. Wögenstein and Peter Fuchs
15. In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure
    Christopher Arnette,Jennifer L. Koetsier, Paul Hoover, Spiro Getsios and Kathleen J. Green
16. Functional Analysis of Periplakin and Envoplakin, Cytoskeletal Linkers and Cornified Envelope Precursor Proteins
    Veronika Boczonadi and Arto Määttä
17. Microtubule-Actin Crosslinking Factor 1: Domains, Interaction Partners and Tissue-Specific Functions
    Dmitry Goryunov and Ronald K. H. Liem
18. Functional and Genetic Analysis of Neuronal Isoforms of BPAG1
    Anisha Lynch-Godrei and Rashmi Kothary
19. "Functional and Genetic Analysis of Spectraplakins in Drosophila
    Ines Hahn, Matthew Ronshaugen, Natalia Sánchez-Soriano and Andreas Prokop
20. Functional and Genetic Analysis of VAB-10 Spectraplakin in C. Elegans
    Christelle Gally, Huimin Zhang and Michel Labouesse
    Part IV: Functional and Genetic Analysis of Lamin-associated proteins
21. Tagged Chromosomal Insertion Site (TCIS) System, a Method to Study Lamina Associated Chromatin
    Jennifer C. Harr and Karen L. Reddy
22. Lamin Binding Proteins in Caenorhabditis Elegans
    Agnieszka Dobrzynska, Peter Askjaer and Yosef Gruenbaum
23. Pathologically-Relevant Prelamin A Interactions with Transcription Factors
    Arantza Infante and Clara I. Rodríguez
24. MCLIP Detection of Novel Protein-Protein Interactions at the Nuclear Envelope
    Mohammed Hakim Jafferali, Ricardo A. Figueroa and Einar Hallberg
25. Preparation of the Human Cytomegalovirus Nuclear Egress Complex and Associated Proteins
    Mayuri Sharma, Jeremy P. Kamil and Donald M. Coen