Ion Channels: Channel Biochemistry, Reconstitution, and Function: Volume 652

Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic. Each chapter is written by an international board of authors.

Autorentext
Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

Inhalt
Preface Daniel L. Minor Jr. and Henry M. Colecraft * Structure/function relationships *

1. Correlating ion channel structure and function Crina Nimigean
2. TRPV3 Expression and Purification for Structure Determination by Cryo-EM Alexander Sobolevsky
3. Methods to study phosphoinositide regulation of ion channels Tibor Rohacs
4. Recombinant Expression and Purification of Pentameric Ligand-Gated Ion Channels for Cryo-EM Structural Studies * Sudha Chakarpani *
5. Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography Anna Moroni * Structural and chemical methods to assess channel dynamics
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6. Structural Dynamics of Channels and Transporters by High-Speed Atomic Force Microscopy Simon Scheuring
7. Crosslinking for gating motions in GluRs Andrew Plested
8. Single molecule FRET methodology for investigating glutamate receptors Vasanthi Jayaraman
9. Interrogating permeation and gating of Orai channels using chemical modification of cysteine residues Murali Prakriya * Channel reconstitution and studies in bilayers ***
10. A quantitative flux assay for the study of Cl- channels and transporters Alessio Accardi
11. Ion channels reconstitution in lipid bilayers Eleonora Zakharian
12. Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayers Gehard Thiel * Methods to assess oligomerization ***
13. The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture Randy Stockbridge