Isolation and Characterization of Alkaloids from Carica Papaya Linn

The carpaine was isolated from Carica papaya L. leaf, was identified by means of Fourier transform infrared spectroscopy, mass spectrometry, nuclear magnetic resonance, HPLC and was determined by LCMS, The powder of dried leaves was macerated and extracted with Methanol/water/HCl (89:10:1 V/V/V) to produce crude alkaloid. The extract was fractionated on a silica gel column chromatography using methanol/chloroform (5:95 v/v) as the eluate. The major fraction was isolated according to Rf values of thin-layer chromatography (TLC) (silica gel, methanol/chloroform [15:85 v/v]) exposed by Dragendroff's reagent. The extraction procedure, sample preparation, and LCMS conditions were evaluated and optimized. The study was done on pharmacological studies on leaves of Carica Papaya Linn.

Autorentext

Aishwarya Shinde-Shete ha conseguito un B. of Pharm presso il Sanjivani College of Pharmacy, Kopargaon e un Master presso l'Amrutvahini College of Pharmacy Sangamner Maharashtra. Attualmente sta svolgendo un dottorato di ricerca presso l'Università di Poona. Autore di oltre 20 articoli pubblicati su riviste nazionali e internazionali rinomate. È titolare di un Young researcher Award 2021 dell'InSc.