Kinase C Probes

Understanding cellular function is a pivotal aim in the life sciences. Monitoring the interplay of regulatory proteins illuminates cellular function on a molecular level. One powerful method is fluorescence microscopy, which enables the spatio-temporal resolution of cellular events. To this end, several fluorescent reporters can be monitored within a single cell and interdependency between these factors can be determined.This book presents fluorescent reporters for monitoring protein kinase C, a central actor on the stage of cellular signalling. Aspects ranging from probe design to the evaluation of experimental results are discussed. Further, experiments designed for high-throughput screening of new probe variants or PKC-effector molecules are described, including methods for visualizing and judging the observed data. Finally, the probe's capacity for use in multiparameter experiments is demonstrated. This book is complemented by a detailed experimental part including a flexible cloning system for rapid probe development and computer scripts supporting the analysis of experimental data.This book is directed to researchers interested in fluorescence probe development.

Autorentext
Schleifenbaum, Andreas Dr. Andreas Schleifenbaum, Diplom-Chemiker: Study of Chemistry at the LMU Munich, diploma thesis at the UC at Berkeley and PhD at the EMBL Heidelberg.