Kupffer Cells

This volume provides a complete overview of kupffer cell biology, from isolation to phenotyping. Chapters guide readers through methods and protocols on phenotyping, including RNA sequencing, Mass cytometry and 3D microscopy, as well as gene manipulation in vivo, and disease models from fatty liver disease to cancer.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge,Kupffer Cells: Methods and Protocols aims to ensure successful investigation of this growing field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Klappentext

This volume provides a complete overview of kupffer cell biology, from isolation to phenotyping. Chapters guide readers through methods and protocols on phenotyping, including RNA sequencing, Mass cytometry and 3D microscopy, as well as gene manipulation in vivo, and disease models from fatty liver disease to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Kupffer Cells: Methods and Protocols aims to ensure successful investigation of this growing field.

Inhalt

Kupffer Cell and Hepatocytes Isolation by Gradient Centrifugation from a Single Mouse Liver.- Kupffer Cell Isolation from Human Biopsies.- Liver Macrophage Isolation by Flow Cytometry Sorting.- Kupffer Cell Protein Isolation and Detection by Western Blot.- Kupffer Cell mRNA Sequencing.- Kupffer Cell and Monocyte-derived Macrophage Identification by Immunofluorescence on FFPE Mouse Liver Sections.- Sample Preparation of Optically Cleared Liver Tissue to Identify Liver Macrophages using 3D Microscopy.- Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer cells in mouse liver.- Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model.- Kupffer Cell Characterization by Mass Cytometry.- Enzymatic Quantification of Liver Lipids after Folch Extraction.- In vivo Investigation of High Fat Diet Induced Hepatic Lipid Dysfunctions.- An Obesogenic Dietary Mouse Model of Nonalcoholic Steatohepatitis.- Generating Hepatocellular Carcinoma in Mice By Expressing N-RasG12V Accompanied with Trp53 Silence Using A Transposon-based System Delivered via Hydrodynamic Ail Vein Injection.- Mouse Model of Alcoholic Steatohepatitis.