Label-Free Biosensor Methods in Drug Discovery

This volume explores label-free biosensors, advantageous in part because this technology bypasses the need of labels, reporters, and cell engineering, all of which are common to labeled techniques but may introduce artifacts in assay results. Addressing several fundamental and practical aspects as to how to implement label-free methods in the drug discovery process, this book covers a wide range of topics, including binding kinetics determination, fragment screening, antibody epitope mapping, protein-protein interaction profiling and screening, receptor pathway deconvolution, drug pharmacology profiling and screening, target identification, drug toxicity assessment, and physical phenotype profiling and diagnostics based on various cellular processes such as cell adhesion, migration, invasion, infection, and inflammation. As part of the Methods in Pharmacology and Toxicology series, chapters aim to provide key detail and implementation advice to aid with progress in the lab.Practical and thorough, Label-Free Biosensor Methods in Drug Discovery provides a new avenue for rapid access to a focused collection of highly regarded contributions in the field.

Assists academic and industrial researchers involved in drug and probe discovery to adopt label-free techniques Provides fundamental and practical methods from expert researchers Includes tips and key implementation advice to aid in translating techniques to your own lab

Inhalt

Label-Free Technologies: Which Technique to Use and What to Watch Out For!.- Label-Free Cell Phenotypic Profiling and Screening: Techniques, Experimental Design, and Data Assessment.- Surface Plasmon Resonance for Therapeutic Antibody Characterization.- Label-Free Cell-Based Biosensor Methods in Drug Toxicology Analysis.- Kinetics Characterization of Ligand-Receptor Interactions Using Oblique-Incidence Reflectivity Difference Method.- Label-Free Inhibition in Solution Assays for Fragment Screening.- Silicon Photonic Micro-Ring Resonators for Drug Screening and Kinetic Analysis.- Optical Waveguide Light-Mode Spectroscopy for Ion Channel Profiling.- Label-Free Profiling of Endogenous Receptor Responses in Primary Isolated Cardiac Cells.- Surface Plasmon Resonance to Study Cell Signaling and GPCR Functional Selectivity in Live Cells.- Triple-Addition Label-Free Assays for High Throughput Screening of Muscarinic M1 Receptor Agonists, Antagonists, and Allosteric Modulators.- Multiplexing Label-Free and Florescence-Based Methods for Pharmacological Characterization of GPCR Ligands.- Label-Free Cell Phenotypic Identification of Active Compounds in Traditional Chinese Medicines.- Use of the Quartz Crystal Microbalance with Dissipation Monitoring for Pharmacological Evaluation of Cell Signaling Pathways Mediated by Epidermal Growth Factor Receptors.- Profiling Sodium-Dependent Phosphate Transporter NaPi-IIb with Resonant Waveguide Grating Biosensor.- Label-Free Impedance Measurements for Profiling Drug-Induced Cardiotoxicity.- Digital Holographic Imaging for Label-Free Phenotypic Profiling, Cytotoxicity, and Chloride Channels Target Screening.- Label-Free Profiling of Cell Adhesion: Determination of the Dissociation Constant for Native Cell Membrane Adhesion Receptor-Ligand Interaction.- Label-Free Impedance-Based Monitoring of Cell Migration and Invasion.- Infrared Surface Plasmon Spectroscopy Decodes Early Processes in Epithelial Host Cells Upon Enteropathogenic Escherichia coli Infection.- Surface Plasmon Resonance for Clinical Diagnosis of Type I Allergy.