LC-MS in Drug Analysis

This Methods in Molecular Biology(TM) volume covers LC-MS screening for drugs of abuse, as well as targeted measurement of analytes including abused drugs, toxic compounds, and therapeutic agents. Includes step-by-step protocols, tips and troubleshooting notes.

Liquid-Chromatography-Mass-Spectrometry procedures have been shown to be successful when applied to drug development and analysis. LC-MS in Drug Analysis: Methods and Protocols provides detailed LC-MS/MS procedures for the analysis of several compounds of clinical significance. The first chapters provide the reader with an overview of mass spectroscopy, its place in clinical practice, its application of MS to TDM and toxicology, and the merits of LC-MS(/MS) and new sample preparation techniques. The following chapters discuss different approaches to screening for drugs of abuse and for general unknowns, as well as targeted measurement of specific analytes or classes of analytes including abused drugs, toxic compounds, and therapeutic agents. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, LC-MS in Drug Analysis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

An Introduction to Drug Testing The Expanding Role of Mass Spectrometry.- LC-MS vs. GC-MS, Online Extraction Systems, Advantages of Technology for Drug Screening Assays.- LC-MS/MS Techniques for High Volume Screening of Drugs of Abuse and Target Drug Quantitation in Urine/Blood Matrices.- Benzodiazepines and Metabolites from Biological Fluids by Liquid Chromatography Electrospray Tandem Mass Spectrometry.- Opiate Screening and Quantitation in Urine/Blood Matrices using LC-MS/MS Techniques.- Synthetic Opioid Analysis by LC-MS/MS.- The Determination of Cannabinoids Using Liquid Chromatography with Mass Spectrometric Detection.- Cocaine and Metabolites by LC-MS/MS.- Quantitation of Amphetamine Type Stimulants by LC-MS/MS.- Detection of Prohibited Substances by Liquid Chromatography Tandem Mass Spectrometry for Sports Doping Control.- LC-MS/MS Screen for Xenobiotics and Metabolites.- Qualitative Identification of Rodenticide Anticoagulants by LC-MS/MS.- Hypoglycemic Agent Screening by LC-MS/MS.- LC-MS/MS Method for the Detection of Common Laxatives.- Simultaneous Determination of Cyclosporine, Sirolimus and Tacrolimus in Whole Blood Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).- Quantification of Tricyclic Antidepressants Using UPLC-MS/MS.- Quantitation of First- and Second-Generation Antipsychotics by LC-MS/MS.- Analysis of Selected Anticonvulsants by High Performance Liquid Chromatography-Tandem Mass Spectrometry.- Therapeutic Drug Monitoring of Tamoxifen Using LC-MS/MS..