Macromolecular Crystallography Protocols, Volume 1

Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators.

Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.

Includes supplementary material: sn.pub/extras

Inhalt
A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag.- Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project.- Baculoviral Expression of an Integral Membrane Protein for Structural Studies.- Protein Engineering.- Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems.- How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals.- Screening and Optimization Methods for Nonautomated Crystallization Laboratories.- Improving Marginal Crystals.- Optimization Techniques for Automation and High Throughput.- Three-Dimensional Crystallization of Membrane Proteins.- Crystallization of Protein-DNA Complexes.- Preparation and Crystallization of RNA.- Crystallization of RNA-Protein Complexes.