Maize

This volume discusses techniques used for the molecular characterization of maize. This book is divided into 4 parts: cell, tissue, and organ culture and maize transformation; gene silencing and generation of mutant populations; plant gene expression; and plant metabolic networks. The chapters cover a range of topics, such as growing and propagating maize in the laboratory, greenhouse and field studies, screening mutagenic population, characterizing the genome, describing protein and metabolic regulatory networks, and generating transgenic plants for gene knock-out and over expression purposes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Maize: Methods and Protocols is a valuable resource for everyone who is interested in maize research.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Use of In Vitro Kernel Culture to Study Maize Nitrogen and Carbohydrate Metabolism.- Agrobacterium- and Biolistic-Mediated Transformation of Maize B104 Inbred.- Advances in Agrobacterium-Mediated Maize Transformation.- A Brief History of Promoter Development for Use in Transgenic Maize Applications.- Deletion Mutagenesis and Identification of Causative Mutations in Maize.- Selective Knockdowns in Maize by Sequence-Specific Protein Aggregation.- Virus-Induced Gene Silencing in Maize with a Foxtail mosaic virus Vector.- Use of a Virus Gene Silencing Vector for Maize Functional Genomics Research.- Laser-Capture Microdissection of Maize Kernel Compartments for RNA-Seq-Based Expression Analysis.- Ribosome Profiling in Maize.- Detection of DNA Methylation by Whole-Genome Bisulfite Sequencing.- Plant Gene Regulation Using Multiplex CRISPR-dCas9 Artificial Transcription Factors.- Cellular and Sub-Cellular Level Localization of Maize Lipids and Metabolites Using High-Spatial Resolution MALDI Mass Spectrometry Imaging.