Measuring Biological Responses with Automated Microscopy: Volume 414

Klappentext The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. Zusammenfassung Part of the "Methods in Enzymology" series! this volume is intended for researchers and reviewers. Inhaltsverzeichnis Green Fluorescent Protein Sensors for High-Content Analysis of the Cell Cycle Fluorescence-Based Screening for Epigenetic Modulators of Assays for Nuclear Receptor Modulators Using Fluorescently Tagged Proteins Ligand-Independent Translocation Assay: An Enabling Technology for Screening Orphan G Protein-Coupled Receptors by Arrestin Recruitment Screening of Known G Protein-Coupled Receptors by Arrestin Translocation Imaging Assays for G Protein-Coupled Receptor Internalization: Application to High-Throughput Screening Confocal Microscopy for ß-Arrestin-Green Fluorescent Protein Translocation G Protein-Coupled Receptor Assays Using the Evotec Opera Protein-Coupled Receptor Internalization Assays in the High-Content Screening Format for Activators of the Wingless Type/Frizzled Pathway by Automated Fluorescent Microscopy Live Cell, Image-Based Approach to Understanding the Enzymology and Pharmacology of 2-Bromopalmitate and Palmitoylation High-Throughput Microscopy Analyses of Nuclear Receptor and Coregulator Function Individual Proteins in Living Cells Using Single Quantum Dot Imaging and Application of Automatic High-Resolution Light Microscopy for Cell-Based Screens Sensors for High-Content Cellular Analysis Assays Using Primary Endothelial Cells to Study Multiple Steps in Inflammation and Implementation of Multiplexed Cell-Based Imaging Assays Screening for Modulators of Stem Cell Differentiation Kinetic Calcium Imaging in Drug-Sensitive and Drug-Resistant Human Breast Cancer Cells and Analysis of Calcium Signaling in Heterogeneous Cell Cultures Analysis of Inflammatory Signaling Pathways Using a High-Content Imaging System and Characterization of a Stable MK2-EGFP Cell Line and Subsequent Development of a High-Content Imaging Assay on the Cellomics ArrayScan Platform to Screen for p38 Mitogen-Activated Protein Kinase Inhibitors and Implementation of Three Mitogen-Activated Protein Kinase (MAPK) Signaling Pathway Imaging Assays to Provide MAPK Module Selectivity Profiling for Kinase Inhibitors: MK2-EGFP Translocation, c-Jun, and ERK Activation Development and Case History of a 32K-Biased Library High-Content MK2-EGFP Translocation Screen to Identify p38 Mitogen-Activated Protein Kinase Inhibitors on the ArrayScan 3.1 Imaging Platform Classification Using Image-Based Cellular Phenotypes Screening: Emerging Hardware and Software Technologies Infrastructure for High-Throughput Microscopy: Instrumentation, Informatics, and Integration Translocation Assays: Key Tools for Accessing New Biological Information with High-Throughput Microscopy Screening of Functional Genomic Libraries Protein-Based Cellular Assays Analyzed by...

Zusammenfassung
Part of the "Methods in Enzymology" series, this volume is intended for researchers and reviewers.

Inhalt

Green Fluorescent Protein Sensors for High-Content Analysis of the Cell Cycle

Fluorescence-Based Screening for Epigenetic Modulators

of Assays for Nuclear Receptor Modulators Using Fluorescently Tagged Proteins

Ligand-Independent Translocation Assay: An Enabling Technology for Screening Orphan G Protein-Coupled Receptors by Arrestin Recruitment

Screening of Known G Protein-Coupled Receptors by Arrestin Translocation

Imaging Assays for G Protein-Coupled Receptor Internalization: Application to High-Throughput Screening

Confocal Microscopy for ß-Arrestin-Green Fluorescent Protein Translocation G Protein-Coupled Receptor Assays Using the Evotec Opera

Protein-Coupled Receptor Internalization Assays in the High-Content Screening Format

for Activators of the Wingless Type/Frizzled Pathway by Automated Fluorescent Microscopy

Live Cell, Image-Based Approach to Understanding the Enzymology and Pharmacology of 2-Bromopalmitate and Palmitoylation

High-Throughput Microscopy Analyses of Nuclear Receptor and Coregulator Function

Individual Proteins in Living Cells Using Single Quantum Dot Imaging

and Application of Automatic High-Resolution Light Microscopy for Cell-Based Screens

Sensors for High-Content Cellular Analysis

Assays Using Primary Endothelial Cells to Study Multiple Steps in Inflammation

and Implementation of Multiplexed Cell-Based Imaging Assays

Screening for Modulators of Stem Cell Differentiation

Kinetic Calcium Imaging in Drug-Sensitive and Drug-Resistant Human Breast Cancer Cells

and Analysis of Calcium Signaling in Heterogeneous Cell Cultures

Analysis of Inflammatory Signaling Pathways Using a High-Content Imaging System

and Characterization of a Stable MK2-EGFP Cell Line and Subsequent Development of a High-Content Imaging Assay on the Cellomics ArrayScan Platform to Screen for p38 Mitogen-Activated Protein Kinase Inhibitors

and Implementation of Three Mitogen-Activated Protein Kinase (MAPK) Signaling Pathway Imaging Assays to Provide MAPK Module Selectivity Profiling for Kinase Inhibitors: MK2-EGFP Translocation, c-Jun, and ERK Activation

Development and Case History of a 32K-Biased Library High-Content MK2-EGFP Translocation Screen to Identify p38 Mitogen-Activated Protein Kinase Inhibitors on the ArrayScan 3.1 Imaging Platform

Classification Using Image-Based Cellular Phenotypes

Screening: Emerging Hardware and Software Technologies

Infrastructure for High-Throughput Microscopy: Instrumentation, Informatics, and Integration

Translocation Assays: Key Tools for Accessing New Biological Information with High-Throughput Microscopy

Screening of Functional Genomic Libraries

Protein-Based Cellular Assays Analyzed by Laser-Scanning Microplate Cytometry in 1536-Well Plate Format

Measurements of Biochemical Responses Using the Plate::Vision Multimode 96 Minilens Array Reader

Cell Biology Based on High-Content Screening

Autofocus Methods for Automated Microscopy

Lifetime Imaging Microscopy: Two-Dimensional Distribution Measurement of Fluorescence Lifetime

Author Index

Subject Index