Meiosis

This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Detailedand practical, Meiosis, Second Edition will prove to be invaluable to biologists, geneticists, biochemists, and anyone investigating meiosis, recombination, and cellular differentiation.

Includes cutting-edge methods and protocols for meiosis research Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in Saccharomyces cerevisiae.- Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein-Binding in S. pombe.- Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast.- Ribosome Profiling for the Analysis of Translation During Yeast Meiosis.- Selection of G1 Phase Yeast Cells for Synchronous Meiosis and Sporulation.- Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora.- High Throughput Screening to Identify Regulators of Meiosis-Specific Gene Expression in Saccharomyces cerevisiae.- Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast.- In Vivo Imaging of Budding Yeast Meiosis.- Sequential Immunofluorescent Light Microscopy and Electron Microscopy of Recombination Nodules During Meiotic Prophase I.- Flow Cytometry for theIsolation and Characterization of Rodent Meiocytes.- Imaging of Chromosome Dynamics in Mouse Testis Tissue by Immuno-FISH.- Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy.- Live Imaging of Meiosis I in Late Stage Drosophila melanogaster Oocytes.- Microscopy Methods for Analysis of Spindle Dynamics in Meiotic Drosophila Spermatocytes.- Drosophila Male Meiosis.- Analysis of Chromatin Dynamics During Drosophila Spermatogenesis.- Modeling of Meiotic Crossover Interference as Inspired by the Beam-Film Model.- A Computational Approach to Study Gene Expression Networks.