Metabolons and Supramolecular Enzyme Assemblies

Metabolons and Supramolecular Enzyme Assemblies, Volume 617 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Dynamic plant metabolons, TCA cycle metabolons, the Chemotactic assembly of metabolons, Repurposing peroxisomes for metabolic engineering, Repurposing yeast mitochondria for metabolic engineering, Repurposing plant compartments for metabolic engineering, Protein scaffolds for pathway co-localization on lipid droplets, Engineered enzyme assemblies for metabolic engineering, NRPS assembly lines and P450 interactions, and much more.

Autorentext
Claudia Schmidt-Dannert is at the University of Minnesota, USA Maureen Quin works at the University of Minnesota, USA

Inhalt

1. Molecular snapshots of dynamic membrane-bound metabolons
   Jean-Etienne Bassard and Tomas Laursen
   2. Tricarboxylic acid metabolon
   Fei Wu and Shelley Minteer
   3. Metabolon formation by chemotaxis
   Xi Zhao and Ayusman Sen
   4. Cloning, expression, and purification of intact polyketide synthase modules
   Laurence Maschio, Alice E. Parnell, Nicholas R. Lees, Christine L. Willis, Christiane Schaffitzel, James E.M. Stach and Paul R. Race
   5. Repurposing peroxisomes for microbial synthesis for biomolecules
   Jiaoqi Gao and Yongjin J. Zhou
   6. Studying trans-acting enzymes that target carrier protein bound amino acids during nonribosomal peptide synthesis
   Anja Greule, Louise K. Charkoudian and Max J. Cryle
   7. Cargo encapsulation in bacterial microcompartments: Methods and analysis
   Taylor Nichols, Nolan Kennedy and Danielle Tullman-Ercek
   8. Bacterial bioreactors: Outer membrane vesicles for enzyme encapsulation
   Kendrick B. Turner, Scott N. Dean and Scott A. Walper
   9. TXTL-based approach to synthetic cells
   Jonathan Garamella, David Garenne and Vincent Noireaux
   10. Cell-surface display of designer cellulosomes by Lactobacillus plantarum
   Yonit Ben-David, Sarah Morais, Johanna Stern, Itzhak Mizrahi and Edward A. Bayer
   11. Enzyme colocalization in protein-based hydrogels
   Louis Lancaster, Beyza Bulutoglu, Scott Banta and Ian Wheeldon
   12. DNA binding adaptors to assemble proteins of interest on DNA scaffold
   Eiji Nakata, Huyen Dinh, Thang Minh Nguyen and Takashi Morii
   13. Protein-based scaffolds for enzyme immobilization
   Guoqiang Zhang, Sarah Schmidt-Dannert, Maureen Quin and Claudia Schmidt-Dannert
   14. Artificial scaffolds for enhanced biocatalysis
   Qing Sun, Shen-Long Tsai, and Wilfred Chen
   15. On-pot and cell-free biocatalysis using coimmobilized enzymes on advanced materials
   Fernando López-Gallego
   16. Combinatorial biosynthesis of small molecules in plants: Engineering strategies and tools
   Iryna Gerasymenko, Yuriy Sheludko, Sabine Fräbel, Agata Staniek and Heribert Warzecha
   17. Insider information on successful covalent protein coupling with help from SpyBank
   Anthony H. Keeble and Mark Howarth
   18. EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors
   Sally Jones, Andrea Martella and Yizhi Cai