Metastasis

This volume provides the most recent developments and methodologies on metastatic process, formation, and detection. Chapters guide readers through functional metastasis in vitro assays, non-mouse and mice metastasis models, methods for imaging metastasis, analyzing the tumor microenvironment, senescence and inflammation with respect to metastasis, methods to investgate the premetastatic/ metastatic, detecting biomarkers in patient, and bioinformatics to simulate the metastatic process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Metastasis: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Cancer Cell Invasion and Metastasis in Zebrafish Models (Danio rerio).- The Chicken Egg Chorionallantois Membrane (CAM) model as an in vivo Method for the Investigation of the Invasion and Metastasis Cascade of Malignant Tumor Cells.- In vitro 3D Models of Tuneable Stiffness.- Patient-derived Xenografts from Solid Tumors (PDX) for Models of Metastasis.- Preparation and Culture of Organotypic Hippocampal Slices for the Analysis of Brain Metastasis and Primary Brain Tumor Growth.- Measuring Mechanical and Adhesive Properties of Single Cells Using an Atomic Force Microscope.- Analysing the Roles of Rho GTPases in Cancer Cell Adhesion to Endothelial Cells under Flow Conditions.- Probing Intravascular Adhesion and Extravasation Of Tumor Cells With Microfluidics.- Real-time Cell Migration Monitoring to Analyze Drug Synergism in the Scratch Assay using the IncuCyte system.- Measurement of Metabolites from Migrating Cells.- Matrix Degradation Assay to Measure the Ability of Tumor Cells to Degrade Extracellular Matrix.- Evaluation of Real-Time In Vitro Invasive Phenotypes.- Detection of Tumor Cell-induced Platelet Aggregation and Granule Secretion.- Fatty Acid Driven Polarization of Suppressive Bone Marrow-Derived Macrophages Including Metabolic and Functional Analysis.- From Whole Blood to Isolated Pro-Metastasis Immune Cells: An Ex-Vivo Approach to Isolate and Manipulate Immune Cells Contributing To Tumor Metastasis.- A Bioinformatic Pipeline to Identify Biomarkers for Metastasis Formation from RNA Sequencing Data.- Barcoding Technology for Multiplexed Analysis of Metastatic Ability In Vivo.- In Vivo Assessment of Metastatic Cell Potential in Prostate Cancer.- Isolation of Nucleoli for Characterization of Nucleolar Contents to Uncover Clues to Metastatic Progression.- Analyzing the Role of Proteases in Breast Cancer Progression and Metastasis using Primary Cells from Transgenic Oncomice.- Nuclear Medicine Imaging Procedures in Oncology.- From Operating Table to Laboratory Bench: The path Towards Metastasis Research from the Clinical Setting.