Methods in Protein Design: Volume 523

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers methods in protein design and it has chapters on such topics as protein switch engineering by domain insertion, evolution based design of proteins, and computationally designed proteins.

Klappentext

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers methods in protein design and it has chapters on such topics as protein switch engineering by domain insertion, evolution-based design of proteins, and computationally designed proteins.

Zusammenfassung
Containing quality chapters authored by leaders in the field, this volume covers methods in protein design and includes chapters on such topics as protein switch engineering by domain insertion, evolution based design of proteins, and computationally designed proteins.

Inhalt

1. Computational Design of Novel Protein Binders and Experimental Affinity Maturation
   

2. Mining Tertiary Structural Motifs for Assessment of Designability

3. **Computational Methods for Controlling Binding Specificity **
4. Flexible Backbone Sampling Methods to Model and Design Protein Alternative Conformations
5. OSPREY: Protein Design with Ensembles, Flexibility, and Provable Algorithms

6. Scientific Benchmarks for Guiding Macromolecular Energy Function Improvement

7. **Molecular Dynamics Simulations for the Ranking, Evaluation, and Refinement of Computationally Designed Proteins **
8. Multi-State Protein Design Using CLEVER and CLASSY
9. Using Analyses of Amino Acid Coevolution to Understand Protein Structure and Function
10. Evolution Based Design of Proteins
11. Protein Engineering and Stabilization from Sequence Statistics: Variation and Co-Variation Analysis
12. Enzyme Engineering by Targeted Libraries
13. Generation of High-Performance Binding Proteins for Peptide Motifs by Affinity Clamping
14. Engineering Fibronectin-Based Binding Proteins by Yeast Surface Display
15. Engineering and Analysis of Peptide-Recognition Domain Specificities by Phage Display and Deep Sequencing
16. Efficient Sampling of SCHEMA Chimera Families to Identify Useful Sequence Elements
17. Protein Switch Engineering by Domain Insertion

18. Design of Chimeric Proteins by Combination of Subdomain-Sized Fragments

19. **a-Helix Mimicry with a/ß-Peptides **