MicroRNA Target Identification

This detailed book provides a comprehensive state-of-the-art presentation of all aspects of miRNA target identification, from the prediction of miRNA binding sites on their target molecules to their experimental validation and downstream functional analysis. It also explores methodologies and tools for target prediction and related analysis, as well as tutorials on specific tasks, tools, and analysis pipelines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, MicroRNA Target Identification: Methods and Protocols updates on basic and advanced concepts involving miRNA target identification and delivers an extremely useful guide to specific computational tasks and analysis in miRNA research.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

A Practical Guide to miRNA Target Prediction.- miRandb: A Meta-Database of Online Resources of miRNA and miRNA Targets.- Sfold Tools for MicroRNA Target Prediction.- The Role of Tertiary Structure in MicroRNA Target Recognition.- Protocols for miRNA Target Prediction in Plants.- Improving miRNA-Target Prediction Using CLASH Data.- Integration of Omics Data to Identify Cancer-Related MicroRNA.- Functional Analysis of Genetic Variants and Somatic Mutations Impacting MicroRNA-Target Recognition: Bioinformatics Resources.- Construction and Analysis of miRNA Regulatory Networks.- Identification of Disease-miRNA Networks across Different Cancer Types Using SWitchMiner (SWIM).- Computational Methods to Investigate the Impact of miRNAs on Pathways.- Consensus Target Prediction with Enrichment Analysis for MicroRNAs Harboring Editing Sites and Other Variations.- A Roadmap for the Computational Prediction and Experimental Validation of Competitive Endogenous RNAs.- Investigating miRNA-lncRNA Interactions: Computational Tools and Resources.- Combing the Hairball: Improving Visualization of miRNA-Target Interaction Networks.- Inconsistencies and Limitations of Current MicroRNA Target Identification Methods.- Experimental Validation of MicroRNA Targets: Luciferase Reporter Assay.- Experimental Validation of MicroRNA Targets: Mutagenesis of the Binding Regions.- Experimental Validation of MicroRNA Targets: Analysis of MicroRNA Targets through Western Blotting.