Mitochondrial Gene Expression

This volume details the most recent advancements in the field of mitochondrial gene expression. Chapters guide readers through methods and protocols on mtDNA replication, transcription, and translation to membrane insertion of the mtDNA-encoded protein products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mitochondrial Gene Expression: Methods and Protocols aims to provide complementary approaches and practical guidelines.

Chapter 7 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
In vitro Analysis of mtDNA Replication.- In vivo Analysis of mtDNA Replication at the Single Molecule Level and with High Resolution.- In Vitro Reconstitution of Human Mitochondrial Transcription.- Investigating Mitochondrial Transcriptomes and RNA Processing using Circular RNA Sequencing.- Detection of 5-formylcytosine in Mitochondrial Transcriptome.- Visualization of Mitochondrial RNA Granules in Cultured Cells using 5-Bromouridine Labeling.- Quantitative Proteomics in Drosophila with Holidic Stable Isotope Labeling of Amino Acids in Fruit Flies (SILAF).- Mass Spectrometric Analysis of Mitochondrial RNA Modifications.- mito--Seq: A High-throughput Method for Systematic Mapping of Pseudouridine within Mitochondrial RNA.- High Throughput Detection of mtDNA Mutations Leading to tRNA Processing Errors.- High-throughput Measurement of Mitochondrial RNA Turnover in Human Cultured Cells.- RNA Crosslinking to Analyze the Mitochondrial RNA-binding Proteome.- Visualizing Mitochondrial Ribosomal RNA and Mitochondrial Protein Synthesis in Human Cell Lines.- Mitoribosome Profiling From Human Cell Culture - A High Resolution View of Mitochondrial Translation.- Application of cryo-EM for Visualization of Mitoribosomes.- Sucrose Gradient Sedimentation Analysis of Mitochondrial Ribosomes.- The Analysis of Yeast Mitochondrial Translation.- In situ Studies of Mitochondrial Translation by Cryoelectron Tomography.- Mitochondrial Complexome Profiling.- Blue Native Electrophoresis to Study the Oxphos Complexes.- Identification of Putative Mitochondrial Protease Substrates.