Mitochondrial Regulation

In this volume *expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Written in the highly successful Methods in Molecular Biology* series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mitochondrial Regulation: Methods and Protocols seeks to aid advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry and bioenergetics.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Isolation of Crud Mitochondrial Fraction from Cells.- Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein Analyses of MicroRNA and Protein Expressions.- PCR Based Determination of Mitochondrial DNA Copy Number in Multiple Species.- NAD+ Content and its Role in Mitochondria.- Measuring PGC-1 and its Acetylation Status in Mouse Primary Myotubes.- Measurement of Mitochondrial Oxygen Consumption Rates in Mouse Primary Neurons and Astrocytes.- Determination Of Oxidative Phosphorylation Complexes Activities.- Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Muscle Biopsies from Patients with Mitochondrial Defects.- A Method Aimed at Assessing the Functional Consequences of the Supramolecular Organization of the Respiratory Electron Transfer Chain by Time-resolved Studies.- BN-PAGE-based Approach to Study Thyroid Hormones and Mitochondrial Function.- Detection of UCP1 Protein and Measurements of Dependent GDP-Sensitive Proton Leak in Non-Phosphorylating Thymus Mitochondria.- Exploring Liver Mitochondrial Function By 13c-Stable Isotope Breath Tests. Implications In Clinical Biochemistry.- Following Mitochondria Dynamism: Confocal Analysis of The Organelle Morphology.- Analysis of Pro-apoptotic Protein Trafficking to and From Mitochondria.- Mitophagy and Mitochondrial Balance.