Molecular Diagnostics for Viral Diseases

This book provides up-to-date information on the molecular diagnosis of viral diseases, including COVID19, zika virus, human immunodeficiency virus (HIV), viral hepatitis, meningitis, and human papilloma virus (HPV). It covers global trends, the impact of viruses on society, and the need for molecular diagnostics. Initial chapters include information on popular molecular diagnostic methods and up-to-date strategies like Nucleic Acid Testing (NAT) and immunological methods, with an understanding of the challenges in their development. Further chapters emphasize emerging and next-generation diagnostic tools such as Specific High-sensitivity Enzymatic Reporter un-LOCKing (SHERLOCK), DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR), and Heating Unextracted Diagnostic Samples to Obliterate Nuclease (HUDSON), which boast a sensitivity of up to attomolar. The book also discusses high-end platforms like QIAsymphony. It is supplemented with illustrations and figures to aid conceptual understanding.

The book is relevant for researchers in virology, clinicians involved in the therapy of viral diseases, and professionals in pathology labs interested in learning about advanced molecular diagnostic approaches for viral diseases.

Provides up-to-date information on genome editing-based diagnostic tools for viral diseases Covers limitations of conventional diagnosis of viral diseases Includes figures and illustrations showcasing diverse diagnostic techniques

Autorentext

Aditya Arya, PhD is working as a scientist at the National Institute of Malaria Research, New Delhi, a flagship lab of the Indian Council of Medical Research (ICMR). By training, he is a biochemist with keen interest in redox biology and molecular diagnostics, with experience at some of the prestigious institutions such as Wellcome Trust Sanger Institute, European Molecular Biology Laboratory, etc. He has published more than 40 research papers in peer-reviewed journals in the domain of medical biochemistry. Moreover, he is also a known author for various popular biology textbooks. For the last four years, his contribution in molecular diagnosis of viral diseases such as SARS-CoV-2 has been part of his scientific journey and recognized at the national level.

Mairaj Ahmed Ansari, PhD is currently working as Assistant Professor at Department of Biotechnology, and Deputy Director, Centre for Virology at Jamia Hamdard, New Delhi. He has over two decades of research experience and published more than 35 peer-reviewed research articles in the area of vaccines, virology, and infection biology. He is also running a successful Master s Degree in Medical Virology course at the University. In the past he has worked on development of vaccine candidates against intracellular pathogens like M. tuberculosis and L. monocytogenes. He also studied mechanism and signaling of innate immunity and its evasion, virus entry and DNA-Damage-Response (DDR), Virus-latency and -lytic cycle maintenance of herpesviruses. He is serving as editorial board member in Springer Nature Virology and various other journals. His eminent work on molecular and clinical virology and exploration of newer paradigms such as exosome-based diagnostics and biomarker discovery has been recent thrust areas.

Inhalt

1. Introduction to Molecular Diagnostics and Viral Diseases.- 2. Molecular Diagnosis of COVID-19: Challenge and Pitfalls.- 3. Choice of Samples for Molecular Diagnosis of Viral Disease.- 4. Molecular and Serological Approaches for the Diagnosis of Human Orthoflaviviruses.- 5. Molecular Diagnosis of Human Immunodeficiency Virus (HIV) Infection.- 6. Recent Progress in Molecular Diagnostics of Hepatitis Viruses.- 7. Introduction to Molecular Diagnosis of Meningitis.- 8. Trends in meningitis prevalence and the need for molecular diagnosis.- 9. Molecular Diagnosis of Human Papillomavirus: Current trends and recent approaches in Molecular Diagnosis.- 10. Molecular Diagnostics for Mosquito-Borne Viral Diseases - Challenges and Emerging Concepts.- 11. Molecular Diagnosis of Enterovirus: Concepts and Challenges.- 12. Advanced Molecular Techniques in Diagnosis of Viral Diseases.- 13. Molecular Diagnosis beyond Nucleic Acid Testing (NAT).