Molecular investigations on archival tissues

Nowadays there are many available molecular methods
for studying diseases. Archival tissue provides an
extremely valuable material for proper understanding
of pathology of various diseases. A major problem in
molecular analysis of archival tissues is the tissue
degradation that might occur during long storage.
This monograph presents various methods for
efficient extraction of DNA and protocols for
sequencing (detection of gene mutations) from
archival tissues. It also provides protocols for
immunodetection of various proteins on such archival
tissues collected at Gades Institute, Haukeland
University Hospital, Norway in the period 1932 till

1996. In addition this book illustrates the value of
      studies on archival tissues for detecting changes in
      the pathogenesis of diseases with time. In this
      monograph bladder cancer is used as an example for
      such studies. This book could be useful for
      professionals, considering the use of archival
      tissue to study mechanisms of various diseases.

      Autorentext

      Jorunn Litlekalsoy is educated as engineer in analytical chemistry at Norwegian High School of Engineering. She obtained an MPhil in Molecular Biology at University of Bergen in 2005 on establishing methods for molecular analysis of archival tissue stored at Gades Institute. She is continuing this work in a PhD study at Gades Institute.

      Klappentext

      Nowadays there are many available molecular methods for studying diseases. Archival tissue provides an extremely valuable material for proper understanding of pathology of various diseases. A major problem in molecular analysis of archival tissues is the tissue degradation that might occur during long storage. This monograph presents various methods for efficient extraction of DNA and protocols for sequencing (detection of gene mutations) from archival tissues. It also provides protocols for immunodetection of various proteins on such archival tissues collected at Gades Institute, Haukeland University Hospital, Norway in the period 1932 till 1996. In addition this book illustrates the value of studies on archival tissues for detecting changes in the pathogenesis of diseases with time. In this monograph bladder cancer is used as an example for such studies. This book could be useful for professionals, considering the use of archival tissue to study mechanisms of various diseases.