Molecular Recognition of DNA Double Helix

In this book, the molecular recognition of DNA using small molecules is discussed, with a study of the photochemistry of Br U-labeled DNA. The purposes of the study were to develop small molecules for regenerative medicine, to develop a method to detect the recognition site of small molecules, and to detect the most important biological phenomena using the photochemistry of Br U-labeled DNA. The study began with the design and development of small molecules that can induce pluripotency genes. To deal with the important issue of cell permeability of the original compound, a new analogue of the original with improved gene expression was designed and synthesized. Using the photochemistry of Br U-labeled DNA, crucial biological phenomena such as cooperativity between transcription factors were detected. For the first time, the cooperativity was examined by excess electron transfer assay. DNA was also studied very carefully in order to understandthe mechanism of the double-strand break in the UVA micro-irradiation technique. The mechanism of the double strand remained untouched. Nevertheless, the double-strand break mechanism was clearly demonstrated by Hoechst dye, as shown in this book.

Nominated by Kyoto University as an outstanding Ph.D. thesis Benefits readers as the design and synthesis of small molecules as articifial transcriptional activators is explained Motivates readers to use this method as a detection tool for other small molecules by conjugating a pyrene-type electron with rich moiety

Autorentext
Dr. Abhijit SAHAInstitut Curie, Campus Universitaire, Orsay, France

Inhalt

1. Overview of DNA minor groove binding synthetic small molecules and photochemistry of Br U substituted DNA.- 2. Synthesis and Biological Evaluation of Targeted Transcriptional Activator with HDAC8 Inhibitory Activity.- 3. Chemical Modification of a Synthetic Small Molecule Boosts its Biological Efficacy against Pluripotency Genes in Mouse Fibroblast.- 4. Development of a novel photochemical detection technique for the analysis of polyamide binding sites.- 5. Examining cooperative binding of Sox2 on DC5 regulatory element upon complex formation with Pax6 through excess electron transfer assay.- 6.UVA irradiation of Br U-substituted DNA in the presence of Hoechst 33258.