Molecular Typing of Blood Cell Antigens

This volume aims to bring together a variety of protocols useful for DNA-based typing of blood cell antigens. Protocols range from simple approaches with low technical complexity to highly sophisticated modern developments. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Typing of Blood Cell Antigens summarizes contributions from leading scientist in the field DNA typing for blood cell antigens.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Validation Of Genotyping Protocols for Diagnostic Use.- High Resolution Melting Analysis Of Single Nucleotide Polymorphisms.- High-Speed Droplet-Allele-Specific Polymerase Chain Reaction for Genotyping Of Single Nucleotide Polymorphisms.- Blood Grouping Based On Pcr Methods and Agarose Gel Eletrophoresis.- Parallel Donor Genotyping For 46 Selected Blood Group and 4 Human Platelet Antigens Using High-Throughput Maldi-Tof Mass Spectrometry.- Pcr With Sequence-Specific Primers for Typing of Diallelic Blood Groups.- High-Resolution Melting Analysis for Genotyping Duffy Blood Group Antigens.- Molecular Rhd-Rhce Analysis by Multiplex Pcr of Short Fluorescent Fragments.- Microarrays in Blood Group Genotyping.- Next Generation Sequencing for Antenatal Prediction of Kel1 Blood Group Status.- 454-Sequencing™ For The Kel, Jr And Lan Blood Groups.- Non-Invasive Prenatal Blood Group Genotyping.- Genotyping Of Human Platelet Antigens By Beadchip Microarray Technology.- Sequence-Based Typing For Platelet Alloantigens.- Miniaturized Technology For Dna Typing: Cassette Pcr.- Geno- And Phenotyping Of Human Neutrophil Antigens.- Allelic Discrimination by Taqman-Pcr For Genotyping of Human Neutrophil Antigens.- Novel Approaches And Technologies in Molecular Hla Typing.- Luminex-Based Methods in High Resolution Hla Typing.- In Silico Hla Typing Using Standard Rna-Seq Sequence Reads.