Mouse Models for Drug Discovery

With genetic engineering, systems explored in this book now exist allowing for the simple, efficient, and near universally precise genetic manipulation directly in any organism, including the mouse. Herein, these models are applied to a wide field of disease areas, including diabetes, cardiovascular disease, skin disorders, cancer, neurodegenerative and neuromuscular diseases, retinal disorders, as well as various behavioral models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and fully updated, Mouse Models for Drug Discovery: Methods and Protocols, Second Edition serves to equip the reader with an extensive overview of techniques to utilize the many possibilities of mice in the drug development process.

Incudes fully updated cutting-edge methods and protocols for using mouse models in drug research Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Klappentext
With genetic engineering, systems explored in this book now exist allowing for the simple, efficient, and near universally precise genetic manipulation directly in any organism, including the mouse. Herein, these models are applied to a wide field of disease areas, including diabetes, cardiovascular disease, skin disorders, cancer, neurodegenerative and neuromuscular diseases, retinal disorders, as well as various behavioral models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and fully updated, Mouse Models for Drug Discovery: Methods and Protocols, Second Edition serves to equip the reader with an extensive overview of techniques to utilize the many possibilities of mice in the drug development process.

Inhalt

Genetically Defined Strains in Drug Development and Toxicity Testing.- Simple, Efficient CRISPR-Cas9 Mediated Gene Editing in Mice: Strategies and Methods.- Mouse Sperm Cryopreservation and Recovery of Genetically Modified Mice.- Generation of Immunodeficient Mice Bearing Human Immune Systems by the Engraftment of Hematopoietic Stem Cells.- Generation of Human Liver Chimeric Mice for the Study of Human Hepatotropic Pathogens.- Human FcRn Transgenic Mice for Pharmacokinetic Evaluation of Therapeutic Antibodies.- A Humanized Mouse Model to Study Human Albumin and Albumin Conjugates Pharmacokinetics.- Germ-Free Mice Model for Studying Host-Microbial Interactions.- Bridging Mice to Men: Using HLA Transgenic Mice to Enhance the Future Prediction and Prevention of Autoimmune Type 1 Diabetes in Humans.- Mouse Models of Type 2 Diabetes Mellitus in Drug Discovery.- Cholesterol Absorption and Metabolism.- Skin Diseases in Laboratory Mice: Approaches to Drug Target Identification and Efficacy Screening.- Chronic Myeloid Leukemia (CML) Mouse Model in Translational Research.- Murine Model for Colitis-Associated Cancer of the Colon.- Mouse Models for Studying Depression-Like States and Antidepressant Drugs.- Experimental Models of Anxiety for Drug Discovery and Brain Research.- Repetitive Behavioral Assessments for Compound Screening in Mouse Models of Autism Spectrum Disorders.- Better Utilization of Mouse Models of Neurodegenerative Diseases in Preclinical Studies: From the Bench to the Clinic.- Neuromuscular Disease Models and Analysis.- Brightfield Imaging and Optical Coherence Tomography of the Mouse Posterior Eye.- Mouse Models as Tools to Identify Genetic Pathways for Retinal Degeneration, as Exemplified by Leber's Congenital Amaurosis.