Multiphoton Microscopy

This volume covers the latest techniques and strategies used in multi-photon excitation (MPE) microscopy. Chapters in this book cover the fundamentals of MPE microscopy as applied to both in vitro and in vivo experimental systems; information on how to combine MPE microscopy with targeted electrophysiological recordings, calcium imaging, and transmembrane voltage imaging; methods to investigate cellular and large-scale neural morphology; signaling in astrocytes; and ways to use MPE microscopy to study the retina. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Comprehensive and thorough, Multiphoton Microscopy is a valuable resource for both expert and novice researchers interested in expanding their knowledge and research in this rapidly developing field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains keynotes and implementation advice from the experts

Inhalt
Building a Two-Photon Microscope is Easy.- Using Multiphoton Imaging for Targeted Electrophysiological Recording and Live Cell Imaging of Fluorescently Labeled Neurons from Isolated Retinas.- Two-Photon Neurotransmitter Uncaging for the Study of Dendritic Integration.- Multiphoton Glutamate Uncaging to Study Structural and Functional Plasticity of Dendritic Spines.- Two-Photon Fluorescence Imaging of Visually Evoked Glutamate Release using iGluSnFR in the Mouse Visual System.- Imaging Neuronal Signal Transduction using Multiphoton FRET-FLIM.- Combining Multiphoton Excitation Microscopy with Fast Microiontophoresis to Investigate Neuronal Signaling.- Multiphoton Excitation Microscopy for Reconstruction and Analysis of Single Neuron Morphology.- Serial Multiphoton Tomography and Analysis of Volumetric Images of the Mouse Brain.- Studying a Light Sensor with Light: Multiphoton Imaging in the Retina.- Multiphoton Ca2+ Imaging of Astrocytes with Genetically Encoded Indicators Delivered by a Viral Approach.- Two-Photon Imaging of Dendritic Calcium Dynamics In Vivo.- Voltage Imaging with ANNINE Dyes and Two-Photon Microscopy.- High-Speed, Random-Access Multiphoton Microscopy for Monitoring Synaptic and Neuronal Activity in 3D in Behaving Animals.