Multiplex Biomarker Techniques

This detailed volume explores the application of multiplex biomarker methods in the critical area of COVID-19 research through state-of-the-art technologies in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging. The book features a series of protocols from labs across the globe employing multiplex molecular approaches, which can be applied to accelerate progress in the research of SARS-CoV-2 and other infectious illnesses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and essential, Multiplex Biomarker Techniques: Methods and Applications for COVID-19 Disease Diagnosis and Risk Stratification serves as a vital resource for researchers in the areas of virology, metabolic diseases, respiratory disorders, as well as to clinical scientists, physicians, pharmacologists, and the healthcare services.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt
Multiplex Technologies in COVID-19 Research, Diagnostics, and Prognostics: Battling the Pandemic.- Multivalent Vaccine Strategies in Battling the Emergence of COVID-19 Variants.- Challenges of Multiplex Assays for COVID-19 Research: A Machine Learning Perspective.- Multiplex Quantitative Polymerase Chain Reaction Diagnostic Test for SARS-CoV-2 and Influenza A/B Viruses.- Multiplex Quantitative Polymerase Chain Reaction Test to Identify SARS-CoV-2 Variants.- NIRVANA for Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and Co-Infections of Multiple Respiratory Viruses.- Quantitative Real-Time RT-PCR Systems to Detect SARS-CoV-2.- Guidance for SARS-CoV-2 RNA-Based Molecular Assay Analytical Performance Evaluations.- A Rapid User-Friendly Lab-on-a-Chip Microarray Platform for Detection of SARS-CoV-2 Variants.- SARS-CoV-2 Host Immunogenetic Biomarkers.- FnCas9 Editor Linked Uniform Detection Assay for COVID-19.- Mass Spectrometry Multiplexed Detection of SARS-CoV-2.- Identification of Circulating Biomarkers of COVID-19 Using MALDI-TOF Mass Spectrometry.- Antibody-Based Affinity Capture Combined with LC-MS Analysis for Identification of COVID-19 Disease Serum Biomarkers.- Liquid Chromatography Mass Spectrometry Analysis of Peripheral Blood Mononuclear Cells from SARS-CoV-2 Infected Patients.- Assay of Fatty Acids and Their Role in the Prevention and Treatment of COVID-19.- Lab-on-a-Chip Immunoassay for Prediction of Severe COVID-19 Disease.- Multiplex Immunoassay for Prediction of Disease Severity Associated with the Cytokine Storm in COVID-19 Cases.- Detection of IgG Antibodies to SARS-CoV-2 and Neutralizing Capabilities Using the Luminex® xMAP® SARS-CoV-2 Multi-Antigen IgG Assay.- Multiplex Testing of the Effect of Statins on Disease Severity Risk in COVID-19 Cases.- Evaluating the Effects of Curcumin on the Cytokine Storm in COVID-19 Using a Chip-Based Multiplex Analysis.- COVID-19 Detection Using the NHS Lateral Flow Test Kit.- Evaluation Protocol for SARS-CoV-2 Serological Assays.- Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs).- Multiplex Testing of Oxidative-Reductive Pathway in Patients with COVID-19 Disease.- Point-of-Care Device for Assessment of Blood Coagulation Status in COVID-19 Patients.- COVID-19 and the Assessment of Coenzyme Q10.- Isolation and Cell Culture of Human Nasopharyngeal Cells: A Model for Testing Immune Response and Antiviral Treatment.- Machine Learning Approaches to Analyze MALDI-TOF Mass Spectrometry Protein Profiles.- A Deep Learning Approach to Identify Chest Computed Tomography Features for Prediction of SARS-CoV-2 Infection Outcomes.- Genomic Surveillance for Monitoring Variants of Concern: SARS-CoV-2 Delta, Omicron, and Beyond.<p