Multiplex Biomarker Techniques

This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due to continuous technical developments and new insights into the high complexity of many diseases. Chapters explore the basic technology platforms being applied in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging, which are currently the methods of choice in multiplex biomarker research. The book also describes the chief medical areas in which the greatest progress has been made and highlight areas where further resources are required. Written for the highly successful Methods in Molecular Biology series, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Multiplex Biomarker Techniques: Methods and Applications serves as an ideal resource for a wide variety of researchers interested in these vital multiplex techniques.

Includes cutting-edge methods and protocols for the study of multiplex biomarker research Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Application of Multiplex Biomarker Approaches to Accelerate Drug Discovery and Development.- The Application of Multiplex Biomarker Techniques for Improved Stratification and Treatment of Schizophrenia Patients.- Multiplex Biomarker Approaches in Type 2 Diabetes Mellitus Research.- LC-MS E , Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics.- Phenotyping Multiple Subsets of Immune Cells In Situ in FFPE Tissue Sections: An Overview of Methodologies.- Identification and Clinical Translation of Biomarker Signatures: Statistical Considerations.- Opportunities and Challenges of Multiplex Assays: A Machine Learning Perspective.- Multiplex Analyses Using Real-Time Quantitative PCR.- Multiplex Analysis Using cDNA Transcriptomic Profiling.- Multiplex Single Nucleotide Polymorphism Analyses.- Pulsed SILAC as a Proteomic Approach for miRNA Targets Identification in Cell Culture.- Blood Bio-SamplingProcedures for Multiplex Biomarkers Studies.- Multiplex Immunoassay Profiling.- Multiplex Sequential Immunopreciptation of Insulin Secretory Granule Proteins from Radiolabeled Pancreatic Islets.- Two Dimensional Gel Electrophoresis of Insulin Secretory Granule Proteins from Biosynthetically-Labeled Pancreatic Islets.- Depletion of Highly Abundant Proteins of the Human Blood Plasma: Applications in Proteomics Studies of Psychiatric Disorders.- Simultaneous Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Analysis of Three Distinct Proteomes.- Selective Reaction Monitoring for Quantitation of Cellular Proteins.- Characterization of a Protein Interactome by Co-Immunoprecipitation and Shotgun Mass Spectrometry.- Using 15 N-Metabolic Labeling for Quantitative Proteomic Analyses.- Multiplex Measurement of Serum Folate Vitamers by UPLC-MS/MS.- UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life.- iTRAQ-Based Shotgun Proteomics Approach for Relative Protein Quantification.- 1 H NMR Metabolomic Profiling of Human and Animal Blood Serum Samples.- Lab-on-a-Chip Multiplex Assays.- Multiplex Smartphone Diagnostics.- Development of a User-Friendly App for Assisting Anticoagulation Treatment.- Multiplex Biomarker Approaches to Enable Point-of-Care Testing and Personalized Medicine.