Muscarinic Receptor: From Structure to Animal Models

This volume provides methods for characterization of muscarinic receptor in crystallography studies that advanced our understanding of structural properties and activation mechanism of muscarinic receptors and are cornerstone in molecular modeling and computerbased approaches to study muscarinic receptors. Muscarinic Receptor: From Structure to Animal Models guides readers through binding techniques, protocols to investigate molecular properties of muscarinic receptors, protocols to study muscarinic receptors in the central nervous system using autoradiography and PET studies and protocols on animals with knock-out and knock-in muscarinic genes to study role of muscarinic receptors in physiology and behavior. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. Concise and easy-to-use, Muscarinic Receptor: From Structure to Animal Models aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Towards the Crystal Structure Determination of Muscarinic Acetylcholine Receptors.- Homology Modeling and Docking Evaluation of Human Muscarinic Acetylcholine Receptors.- Radioligand Binding at Muscarinic Receptors.- Binding Method for Detection of Muscarinic Acetylcholine Receptors in Receptor's Natural Environment.- Use of Antibodies in the Research on Muscarinic Receptor Subtypes.- Allosteric Modulation of Muscarinic Receptors.- Subcellular and Synaptic Localization of Muscarinic Receptors in Neurons Using High-Resolution Electron Microscopic Preembedding Immunogold Technique.- Investigation of Muscarinic Receptors by Fluorescent Techniques.- Autoradiography Assessment of Muscarinic Receptors in the Central Nervous System.- Imaging of Muscarinic Receptors in the Central Nervous System.- Detection of Non-Neuronal Acetylcholine.- Utilization of Superfused Cerebral Slices in Probing Muscarinic Receptor Autoregulation of Acetylcholine Release.- Regulation of Heart Contractility by M2 and M3 Muscarinic Receptors: Functional Studies using Muscarinic Receptor Knockout Mouse.- Muscarinic Receptor Gene Transfections and In Vivo Dopamine Electrochemistry: Muscarinic Receptor Control of Dopamine-Dependent Reward and Locomotion.