Nano Biosensor Designing for Staphylococcus aureus Exotoxin Detection

In this technique, the production of molecular framework and polymer is done using meta acrylic acid monomers, which are formed via covalence connection between meta acrylic acid monomers (MAA) of white polymer. Here also hydrogenic connection between exotoxin amino acid and meta acrylic acid is made that would function as the selective absorption for that. Then in the second stage, based on the bacterial antibody connection to nanoparticel, a sensor was used. In this part of the research, as the basis for absorption for the recognition of bacterial toxin, medium sized silica nanoparticles of 10 nano meter in form of solid powder were utilized with Notrino brand. Then the suspension produced from agent-linked nanosilica which was connected to bacterial antibody was positioned near the samples of distilled water, that were contaminated with Staphylococcus Aureus bacterial toxin with the density of 10-3, so that in case any toxin exists in the sample, a connection between toxin antigen and antibody would be formed. Finally, the light absorption related to the connection of antigen to the particle attached antibody was measured using spectrophotometry.

Autorentext

Dr H.Ahari is Assistant Professor and Member of Scientific Board on Food science and Nutrition Faculty (Science and Research Branch,Islamic Azad university)He Holds DVM and PhD Degree in Food Hygiene.He published 8 Books and more than 32 Articles,6 Patents. International Food Lead Auditor of (WFSO) ,International Association for Food Protection