Nanomaterial Interfaces in Biology

This book covers protocols describing synthesis, fabrication, and construction of bio-nanomaterial interfaces, characterization protocols of bio-nanomaterial interfaces, and applications which utilize the bio-nanomaterial interfaces.

The intersection of nanotechnology with biology has given rise to numerous ideas for new ways to use nanotechnology for biological applications. Nanomaterials possess unique size- and material-dependent properties which make them attractive for improving regular biomedical fields, such as drug delivery, imaging, therapy, and diagnostics. Divided into three convenient sections, Nanomaterial Interfaces in Biology: Methods and Protocols covers protocols describing synthesis, fabrication, and construction of bio-nanomaterial interfaces, characterization protocols of bio-nanomaterial interfaces, and applications which utilize the bio-nanomaterial interfaces. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible Nanomaterial Interfaces in Biology: Methods and Protocols will serve the new and emerging scientific community, enabling new capabilities and technologies that were not previously possible in medicine and biology.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Preparation of 2 nm Gold Nanoparticles for In vitro and In vivo Applications.- DNA Conjugation to Nanoparticles.- Conjugation of Nanoparticles to Proteins.- Water-Solubilization and Functionalization of Semiconductor Quantum Dots.- Synthesizing and Modifying Peptides for Chemoselective Ligation and Assembly into Quantum Dot-Peptide Bioconjugates.- Reliable Methods for Silica Coating of Au Nanoparticles.- Surface Modifications by Polymers for Biomolecule Conjugation.- Functionalization Protocols of Silicon Micro-Nano-Mechanical Biosensors.- Stability and Aggregation Assays of Nanoparticles in Biological Media.- Electrochemical Measurements of DNA Melting on Surfaces.- Formation and Characterization of Nanoparticle-Protein Corona.- Electrophoretic Implementation of the Solution-Depletion Method for Measuring Protein Absorption, Adsorption Kinetics, and Adsorption Competition Among Multiple Proteins in Solution.- Hyperspectral Microscopy for Characterization of Gold Nanoparticles in Biological Media and Cells for Toxicity Assessment.- Immunochemistry, Electron Tomography and Energy Dispersive X-Ray Spectroscopy (EDXS) on Cryosections of Human Cancer Cells Doped with Stimuli Responsive Polymeric Nanogels Loaded with Iron Oxide Nanoparticles.- Zwitterion Siloxane to Passivate Silica Against Non-Specific Protein Adsorption.- Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles.- Polyaspartic Acid Coated Iron Oxide Nanoprobes for PET/MRI Imaging.- Ligand Synthesis and Passivation for Silver and Large Gold Nanoparticles for Single-Particle-Based Sensing and Spectroscopy.- Non-Covalent Intracellular Drug Delivery of Hydrophobic Drugs on Au NPs.- Modification of Carbon Nanotubes for Gene Delivery Vectors.- Lipid-Based Nanoparticles as Non-Viral Gene Delivery Vectors.- Stabilizing Gold Nanoparticle Bioconjugates in Physiological Conditions by PEGylation.