Natural Product Biosynthesis by Microorganisms and Plants, Part A: Volume 515

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 3 volumes covering Natural product biosynthesis by microorganisms and plants, it has chapters on such topics as Kinetics of plant sesquiterpene synthases, Terpenoid biosynthesis in fungi, and plant Type III polyketide synthases.

Klappentext

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 3 volumes covering natural product biosynthesis by microorganisms and plants, it has chapters on such topics as kinetics of plant sesquiterpene synthases, terpenoid biosynthesis in fungi, and plant Type III polyketide synthases.

Zusammenfassung
Part of Methods in Enzymology series, this title covers Natural product biosynthesis by microorganisms and plants, it has chapters on such topics as Kinetics of plant sesquiterpene synthases, and plant Type III polyketide synthases.

Inhalt

1. Steady-state kinetic characterization of sesquiterpene synthases by gas chromatography-mass spectroscopy*
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2. **Automating gene library synthesis by structure-based combinatorial protein engineering (SCOPE): examples from plant sesquiterpene synthases
   
3. In planta Transient Expression Analysis of Monoterpene Synthases
   
4. Natural Rubber Biosynthesis in Plants: Rubber Transferase
   
5. Discovery and Characterization of Terpenoid Biosynthetic Pathways of Fungi
   
6. Menaquinone biosyntheses in microorganisms
   
7. Diversity and analysis of bacterial terpene synthases
   
8. *Platensimycin and platencin biosynthesis in Streptomyces platensis, showcasing discovery and characterization of novel bacterial diterpene synthases
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9. **Strategies for Engineering Plant Natural Products:The Iridoid-Derived Monoterpene Indole Alkaloids of Catharanthus roseus
   
10. Discovery and functional analysis of monoterpenoid indole alkaloid pathways in plants***
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11. Biochemical Genomics for Gene Discovery in Benzylisoquinoline Alkaloid Biosynthesis in Opium Poppy and Related Species
    
12. Analysis and modification of ergot alkaloid profiles in fungi
    
13. Engineering of glucosinolate biosynthesis: candidate gene identification and validation
14. *Structure, function and evolutionary analyses of the plant type III polyketide synthase family
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15. Engineering of plant type III polyketide synthases
    
16. Type III polyketide synthases in microorganisms