Neuronal Cell Culture

This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

General Overview of Neuronal Cell Culture.- Considerations for the use of SH-SY5Y Neuroblastoma Cells in Neurobiology.- Cultured cell line models of neuronal differentiation: NT2, PC12, SK-N-MC.- Murine teratocarcinoma-derived neuronal cultures.- Isolation and propagation of primary human and rodent embryonic neural progenitor cells and cortical neurons.- Mouse Enteric Neuronal Cell Culture.- Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications.- Slice culture modeling of CNS viral infection.- Neurospheres and Glial cell cultures: immunocytochemistry for cell phenotyping.- Transfection of Neuronal Cultures.- Lentiviral Transduction of Neuronal Cells.- Compartmentalized Neuronal Cultures.- Quantitative assessment of neurite outgrowth over growth promoting or inhibitory substrats.- Isolation and Culture of Dorsal Root Ganglia (DRG) from Rodents.- Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection.- Fetal Brain Injury Models of Fetal Alcohol Syndrome: Examination of Neuronal Morphologic Condition Using Sholl Assay.