Neuronal Network Analysis

In recent years, technologies used to monitor and manipulate neuronal networks at single-cell resolution have expanded rapidly. This book examines the latest techniques in the field as well as highlighting current limitations and positing future developments.

In the last few years, technologies that allow for monitoring and manipulating neuronal networks at a single cell resolution have been rapidly expanding. In Neuronal Network Analysis: Concepts and Experimental Approaches, experts in the field provide an overview of some of the most advanced techniques for the study of neuronal circuits, highlighting current technical limitations and providing a vision of what is yet to come. Broken into six convenient, interdependent section, this volume covers optical approaches, electrophysiological approaches, both in vitro and in vivo, optogenetics, morpho-functional approaches, and neuronal network analysis in humans. As a volume in the Neuromethods series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

Authoritative and cutting-edge, Neuronal Network Analysis: Concepts and Experimental Approaches will be useful for early career neuroscientists, as well as for experienced neuroscientists, by providing a practical framework for studying the brain as an organ while maintaining an appreciation for its cellular diversity.

Collects the latest technology used on neural network studies Provides a vision of the present and future of circuit neuroscience Includes expert tips and implementation advice to ensure the successful reproduction of the protocols provided

Inhalt

Optical Interrogation of Neural Circuits.- In Vivo Functional Imaging of the Olfactory Bulb at Single Cell Resolution.- Two-Photon Imaging of Neural Activity in Awake, Head-Fixed Mice.- Spatial Light Modulators for Complex Spatio-Temporal Illumination of Neuronal Networks.- Voltage-Sensitive Dye Imaging of Cortical Function In Vivo.- An Introduction to In Vitro Slice Approaches for the Study of Neuronal Circuitry.- Extracellular Recordings of Synaptic Plasticity and Network Oscillations in Hippocampal Slices.- Use of Dynamic-Clamp as a Tool to Reveal the Computational Properties of Single Neurons Embedded in Cortical Circuits.- Fast Perfusion Methods for the Study of Ligand-Gated Ion Channels.- Electrophysiological Approaches for Studying Neuronal Circuits In Vivo.- Determination of Cortical Circuit Function Using Current Source-Density (CSD) Analysis In Vivo.- Intracellular Whole-Cell Patch-Clamp Recordings of Cortical Neurons in Awake Head-Restrained Mice.- Investigating Sleep Homeostasis with Extracellular Recording of Multiunit Activity from the Neocortex in Freely-Behaving Rats.- Analysis of Hippocampal Memory Replay Using Neural Population Decoding.- High Speed Videography of Embodied Active Sensing in the Rodent Whisker System.- Light-Activated Ion Pumps and Channels for Temporally-Precise Optical Control of Activity in Genetically-Targeted Neurons.- Integrated Optogenetic and Electrophysiological Dissection of Local Cortical Circuits In Vivo.- Combining Optical Stimulation with Extracellular Electrophysiology in Behaving Mice.- Morphological Approaches to the Anatomical Dissection of Neuronal Circuits.- The Isotropic Fractionator: A Fast, Reliable Method to Determine Numbers of Cells in the Brain or Other Tissues.- Morpho-Functional Mapping of Cortical Networks inBrain Slice Preparations Using Paired Electrophysiological Recordings.- Combining Transcranial Magnetic Stimulation with Electroencephalography to Study Human Cortical Excitability and Effective Connectivity.- Biophysically Principled Computational Neural Network Modeling of Magneto-/Electro-Encephalography (MEG/EEG) Measured Human Brain Oscillations.