New and Emerging Proteomic Techniques

New Protocols From Expert Users to
Enable Enhanced Protein Analysis

New and Emerging Proteomic Techniques looks to present some of the new and emerging proteomics tools and techniques that enable enhanced protein analyses. The techniques span the entire spectrum of top-down and bottom-up approaches, and are clear example of how proteomics has embraced essentially all techniques that contend with protein analysis. Aimed at both beginners and more experienced practitioners in the field of proteomics, the book offers protocols and know-how from the pioneers and the expert users of each of these techniques, in details that are usually not found in a typical research publication.

Includes supplementary material: sn.pub/extras

Klappentext
Numerous emerging technologies have propelled the growth of proteomics into a wide-ranging discipline with great potential to improve human health through the discovery of new protein biomarkers and therapeutics. In New and Emerging Proteomic Techniques, a panel of leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods described here span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. Where necessary, two or more distinct techniques are provided for a given problem, and advanced bioinformatics approaches are presented for the analysis of complex proteomic data. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and easy to use, New and Emerging Proteomic Techniques offers expert and novice investigators alike today's most promising proteomics techniques for probing the biological processes and understanding the diseases of the human body.

Inhalt
On-Chip Protein Synthesis for Making Microarrays.- RCA-Enhanced Protein Detection Arrays.- Antibody Microarrays Using Resonance Light-Scattering Particles for Detection.- Chemical Proteomics Profiling of Proteasome Activity.- Two-Dimensional Difference Gel Electrophoresis.- Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors.- Surface Plasmon Resonance Imaging Measurements of Protein Interactions With Biopolymer Microarrays.- Surface Plasmon Resonance Mass Spectrometry for Protein Analysis.- High-Throughput Affinity Mass Spectrometry.- Isotope-Coded Affinity Tags for Protein Quantification.- Proteomic Analysis by Multidimensional Protein Identification Technology.- Isolation of Glycoproteins and Identification of Their N-Linked Glycosylation Sites.- N-Glycosylation Analysis Using the StrOligo Algorithm.- MALDI-MS Data Analysis for Disease Biomarker Discovery.- Using the Global Proteome Machine for Protein Identification.