New Trends in Large-Scale Mammalian Cell Culture: Cell Adaptation

This book offers the experiences and improvements made in mammalian cell culture technology. It bridges two scientific cultures, Scientists with no background in cultivation of animal cells and for engineers with a little ground in biology. It was designed to provide you with immediate access to the protocol required every day for animal and mammalian cell lines cultures. It explains the advantages and disadvantages of the methods, different application and gives advice whichever procedure to use. The author supplements the ideas by simple worked examples, making the techniques of scaling up cells by adaptation strategy for pharmaceutical protein production easy to learn. Monitoring cell growth kinetics accompanied by cell metabolism (glucose and glutamine consumption, lactate and ammonia production) and cell imaging established well. It discusses removal of serum, media supplement like insulin and amino acids, osmotic stress and cell morphology. It worth mentioned that, this book open a new gate for biotechnological scientists describing manipulation of productive adherence cell factory in serum medium to grow as suspension cells in serum free medium at high cell density.

Autorentext

Mr. Abdalla A. Elshereef received his BSc in biotechnology, Benha Univ.,and MSc from Zoology Dept., Mansoura Univ.He nominated by the TWAS as one of the top 100 scientists in the field in biovision 2010.He is Assistant Researcher, Microbial and Natural Chemistry Dept., pharmaceutical&drug industries division at National Research Center of Egypt