Next-Generation MicroRNA Expression Profiling Technology

The rapid pace of research in this field continues to drive technical advances that make methods more sensitive and applicable. This volume features many new methodologies described by leading researchers, including reproducible laboratory protocols.

The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years. In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today. This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development. Written in the highly successful Methods in Molecular Biology™ series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.

Covers innovative technologies that are more sensitive, specific, and quantitative, as well as being compatible with a wide range of biospecimens Features tried-and-true lab protocols that are ready to be put into action Includes expert tips and implementation advice to ensure successful results

Inhalt

Controlling miRNA Regulation in Disease.- Introduction to miRNA Profiling Technologies and Cross-Platform Comparison.- Stem-Loop RT-qPCR for MicroRNA Expression Profiling.- Poly(T) Adaptor RT-PCR.- MicroRNA In Situ Hybridization.- Agilent MicroRNA Microarray Profiling System.- miRNA Gene Expression Profiling Using Illumina Universal BeadChips.- MicroRNA Expression Analysis Using the Affymetrix Platform.- Individualized miRNA Assay Panels Using Optically Encoded Beads.- Microfluidic Primer Extension Assay (MPEA).- MicroRNA Profiling Using µParaflo Microfluidic Array Technology.- MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform.- Next Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application.- Methods for Small RNA Preparation for Digital Gene Expression Profiling by Next-Generation Sequencing.- Profiling of Short RNAs Using Helicos Single-Molecule Sequencing.- deepBase: Annotation and Discovery of microRNAs and Other Non-Coding RNAs from Deep Sequencing Data.- PhenomiR: MicroRNAs in Human Diseases and Biological Processes.- miRNA Expression Profiling: From Reference Genes to Global Mean Normalization.- miRNA Data Analysis: Next-Gen Sequencing.- Integrated miRNA Expression Analysis and Target Prediction.- miRNAs in Human Cancer.- Blood-Based miRNA Preparation for Non-Invasive Biomarker Development.