NMDA Receptors

Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

NMDA Receptors in the Central Nervous System.- Quantification of NMDAR Subunit Genes Expression by qRT-PCR.- Genetic and Functional Analysis of GRIN2A in Tumor Samples.- Detection of NMDARs Antibodies in Encephalitis.- MagnetofectionTM of NMDA Receptor Subunits GluN1 and GluN2A Expression Vectors in Non-Neuronal Host Cells.- Transfection in Primary Cultured Neuronal Cells.- Selective Cell-Surface Expression of Triheteromeric NMDA Receptors.- Functional Analysis of Recombinant Channels in Host Cells using a Fast Agonist Application System.- GluN2B Subunit Labeling with Fluorescent Probes and High-Resolution Live Imaging.- Design of Light-Sensitive NMDARs by Genetically Encoded Photo-Cross-Linkers.- Gene Targeted Mice with Conditional Knock-In (-Out) of NMDAR Mutations.- Electrophysiological Investigation of NMDA Current Properties in Brain Slices.- Analysis of Functional NMDA Receptors in Astrocytes.- GluNs Detection and Functions in Microglial Cells.- NMDA ReceptorActivity in Circulating Red Blood Cells: Methods of Detection.- NMDA Receptors as Voltage Sensors.- Development of a Computational Approach/Model to Explore NMDA Receptors Functions.