Nucleosomes, Histones and Chromatin Part A: Volume 512

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering nucleosomes, histones and chromatin, it has chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins.

Klappentext

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering nucleosomes, histones and chromatin, it has chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins.

Zusammenfassung
Part of the Methods in Enzymology series, this title covers Nucleosomes, Histones & Chromatin and has chapters on Methods applied to the High resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins.

Inhalt

1. Methods applied to the study of Protein Arginine Methylation 2. TBC

   3. Genome-wide mapping of nucleosomes in budding yeast

   4. High resolution identification of intra- and interchromosomal DNA interactions by 4C technology

   5. TBC

   6. High throughput assays to identify small-molecule inhibitors of chromatin 'reader' proteins

   7. Peptide microarrays to interrogate the âEUR histone codeâEUR(TM)

   8. Preparation of Drosophila cells from different stages of the cell cycle for ChIP using centrifugal elutriation and FACS

   9. Analysis of Single and Combinatorial Histone PTM Patterns by High Resolution Mass Spectrometry

   10. Measuring nucleosome turnover dynamics genome-wide

   11. Comparative mRNA expression-profiling of chromatin factors

   12. High-Throughput MAPit Single-Molecule Footprinting

   13. Genome-wide in vitro reconstitution

   14. Quantitating interactions of nuclear factors with chromatin

   15. Biochemical assay for histone H2AZ (Htz1) replacement by the yeast SWR1 chromatin remodeling complex 16. TBC

   16. TBC **