Odorant Binding and Chemosensory Proteins

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

Autorentext
Paolo Pelosi is at AIT Austrian Institute of Technology GmbH, Austria Wolfgang Knoll is at AIT Austrian Institute of Technology GmbH, Austria

Inhalt

1. Genome mining and sequence analysis of chemosensory soluble proteins in arthropods*Joel Vizueta, Paula Escuer, Alejandro Sánchez-Gracia and Julio Rozas

   ---

2. Reconstructing protein coding sequences from ancient DNA* Michael Hofreiter and Stefanie Hartmann

   ---

3. Bioinformatic, genomic and evolutionary analysis of genes: A case study in Dipteran CSPs *** Jean-François Picimbon

   ---

4. Proteomics of arthropod soluble olfactory proteins* Francesca R. Dani and Giuseppe Pieraccini

   ---

5. Analysis of post-translational modifications in soluble proteins involved in chemical communication from mammals and insects* Andrea Scaloni

   ---

6. Bacterial expression and purification of vertebrate odorant-binding proteins * Marine Brulé, Margot Glaz, Christine Belloir, Nicolas Poirier, Lucie Moitrier, Fabrice Neiers and Loïc Briand

   ---

7. Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography* Anaïs Gaubert, Béatrice Amigues, Silvia Spinelli and Christian Cambillau

   ---

8. Solution structure of insect CSP and OBPs by NMR* Serena Leone, Alessandro Emendato, Roberta Spadaccini and Delia Picone

   ---

9. Stability of OBPs* Nadja Hellmann

   ---

10. Ligand-binding assays with OBPs and CSPs* Chiara D'Onofrio, Valeriia Zaremska, Jiao Zhu, Wolfgang Knoll and Paolo Pelosi

    ---

11. Fine structure immunocytochemistry-An important tool for research on odorant-binding proteins* Rudolf Alexander Steinbrecht

    ---

12. Effect of OBPs on the response of olfactory receptors* Bing Wang, Song Cao, Wei Liu and Guirong Wang

    ---

13. Site-directed mutagenesis of odorant-binding proteins* Jiao Zhu, Valeriia Zaremska, Chiara D'Onofrio, Wolfgang Knoll and Paolo Pelosi

    ---

14. Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing* Yusuke Shiota and Takeshi Sakurai

    ---

15. Electrochemical biosensing with odorant binding proteins* Sabine Szunerits, Rabah Boukherroub and Alina Vasilescu

    ---

16. Electronic biosensors based on graphene FETs* Patrik Aspermair, Vladyslav Mishyn, Sabine Szunerits and Wolfgang Knoll

    ---

17. Electronic biosensors based on EGOFETs* Mohammad Y. Mulla, Luisa Torsi and Kyriaki Manoli

    ---

18. Gravimetric biosensors* Khasim Cali, Elena Tuccori and Krishna C. Persaud

    ---

19. Plasmonic biosensors relying on biomolecular conformational changes: Case of odorant binding proteins**

    Anil Bozdogan, Simone Hageneder and Jakub Dostalek