Oral Biology

This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and tissues. The first section contains chapters that discuss proteomic analyses by mass spectrometry and NMR-based metabolomics that can be used to not only study saliva, but also to assess other oral fluids such as gingival crevicular fluid. The second section contains chapters that profile oral microbial communities, quantitative real-time PCR, and adhesion of yeast and bacteria to oral surfaces. The third section deals with a range of approaches that enable the behavior of cells and tissues in both health and disease to be analyzed at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Oral Biology: Molecular Techniques and Applications, Second Edition is a useful resource for oral biologists at all levels (students, early career researchers, and experienced veterans), and it provides a ready reference to new techniques and approaches that can be used to answer numerous specific scientific questions that may lead to a deeper understanding and treatments of oral diseases.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Salivary Diagnostics using Purified Nucleic Acids.- RNA Sequencing Analysis of Salivary Extracellular RNA.- Qualitative and Quantitative Proteomic Analysis of Oral Fluids in Health and Periodontal Disease by Mass Spectrometry.- Antioxidant Micronutrients and Oxidative Stress Biomarkers.- NMR-Based Metabolomics of Oral Biofluids.- Gene Therapy of Salivary Diseases.- The Oral Microbiota in Health and Disease: An Overview of Molecular Findings.- Microbial Community Profiling using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE).- Analysis of 16S rRNA Gene Amplicon Sequences using the QIIME Software Package.- Adhesion of Yeast and Bacteria to Oral Surfaces.- Quantitative Analysis of Periodontal Pathogens by Real-Time Polymerase Chain Reaction.- Methods to Study Antagonistic Activities Among Oral Bacteria.- Natural Transformation of Oral Streptococci by Use of Synthetic Pheromones.- Markerless Genome Editing in Competent Streptococci.- Tools and Strategies for Analysis of Genome-Wide and Gene-Specific DNA Methylation Patterns.- Generating Multiple Base-Resolution DNA Methylomes using Reduced Representation Bisulfite Sequencing.- A Protocol for the Determination of the Methylation Status of Gingival Tissue DNA at Specific CpG Islands.- Genome-Wide Expression Analysis of Periodontal Cells and Tissues.- Differential Expression and Functional Analysis of High-Throughput -Omics Data Using Open Source Tools.- Exploring Genome-Wide Expression Profiles Using Machine Learning Techniques.- Embryonic Explant Culture: Studying Effects of Regulatory Molecules on Gene Expression in Craniofacial Tissues.- Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease.- Fabrication and Characterization of Decullularized Periodontal Ligament Cell Sheet Constructs.- A Method to Isolate, Purify and Characterize Human Periodontal Ligament Stem Cells.- Constructing Tissue Microarrays: Protocols and Methods Considering Potential Advantages and Disadvantages for Downstream Use.- Growing Adipose-Derived Stem Cells under Serum-Free Conditions.- Quantitative Real-Time Gene profiling of Human Alveolar Osteoblasts.- Proteomic Analysis of Dental Tissue Microsamples.- Characterization, Quantification and Visualization of Neutrophil Extracellular Traps.