PCR

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High Quality Content by WIKIPEDIA articles! In molecular biology, the polymerase chain reaction (PCR) is a technique to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. The method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated amplification. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the DNA template is exponentially amplified. PCR can be extensively modified to perform a wide array of genetic manipulations.

Weitere Informationen

  • Allgemeine Informationen
    • GTIN 09786130505967
    • Editor Lambert M. Surhone, Miriam T. Timpledon, Susan F. Marseken
    • Größe H5mm x B220mm x T150mm
    • EAN 9786130505967
    • Format Fachbuch
    • Titel PCR
    • Gewicht 134g
    • Herausgeber Betascript Publishing
    • Anzahl Seiten 88
    • Genre Biologie

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