Plant Membrane Proteomics

This volume provides comprehensive and detailed protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as isolation and purity assessment of membranes from Norway spruce; proteomic analysis of nuclei; analyzing the vacuolar membrane (tonoplast) proteome; isoforms of a thylakoid-bound protein; assay of plasma membrane H+-ATPase in plant tissue under abiotic stresses; and identification and characterization of plant membrane proteins using ARAMEMNON. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Plant Membrane Proteomics: Methodsand Protocols is a valuable resource that promotes the use of plant membrane proteomics to develop the future of the field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Free Flow Zonal Electrophoresis for Fractionation of Plant Membrane Compartments prior to Proteomic Analysis.- Isolation and Purity Assessment of Membranes from Norway Spruce.- Nuclear Proteome: Isolation of Intact Nuclei, Extraction of Nuclear Proteins and 2-DE Analysis.- Identification of Plant Nuclear Proteins Based on a Combination of Flow Sorting, SDS-PAGE, and LC-MS/MS Analysis.- Isolation, Purity Assessment, and Proteomic Analysis of Nuclei.- Proteomic Analysis of Rice Golgi Membranes Isolated by Floating through Discontinuous Sucrose Density Gradient.- Analyzing the Vacuolar Membrane (Tonoplast) Proteome.- Preparation of Membrane Fractions (Envelope, Thylakoids, Grana and Stroma Lamellae) from Arabidopsis Chloroplasts for Quantitative Proteomic Investigations and Other Studies.- Isolation of Intact Thylakoid Membranes from Heterocytes of Filamentous, Nitrogen-Fixing Cyanobacteria.- Targeted Quantification of Isoforms of a Thylakoid-Bound Protein: MRM Method Development.- Sample Preparation for Analysis of the Plant Mitochondrial membrane Proteome.- Plasma Membrane Proteomics of Arabidopsis Suspension Cultured Cells Associated with Growth Phase using Nano-LC-MS/MS.- Mini-Scale Isolation and Preparation of Plasma Membrane Proteins from Potato Roots for LC/MS Analysis.- Assay of Plasma Membrane H+-ATPase in Plant Tissues under Abiotic Stresses.- Absolute Quantification of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters.- MSE for Label-Free Absolute Protein Quantification in Complex Proteomes.- Identification and Characterization of Plant Membrane Proteins using ARAMEMNON.- VANTED: A Tool for Integrative Visualization and Analysis of Omics Data.