Plant Tissue Culture

Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples.

The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists.

Klappentext

Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists.

Zusammenfassung
Provides a diverse learning experience and is appropriate for both university students and plant scientists. This book offers exercises that demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation.

Inhalt

1. History of Plant Cell Culture2. Setup of a Tissue Culture Laboratory3. Media Components and Preparation4. Explant Preparation5. Contamination6. Callus Induction7. Regeneration and Morphogenesis8. Woody Shrubs and Trees9. Haploid Plants from Anther Culture10. Embryo Rescue11. Meristem Culture for Virus-Free Plants12. In Vitro Propagation for Commercial Production of Ornamentals13. Protoplast Isolation and Fusion14. Agrobacterium-Mediated Transformation of Plants

   AppendixI: Useful MeasurementsII: Review of Solution PreparationIII: List of SuppliersIV: Common Plant Tissue Culture Terms