Protein Design

Up-to-Date Protein Design and Engineering Strategies

The aim of Protein Design: Methods and Applications is to account for the most up-to-date protein design and engineering strategies so that readers can undertake their own project with a maximum chance of success. Topics of discussion include: protein design, binding of peptides to target proteins, avoiding protein aggregation while keeping fold stability, and the structural features that should be considered with acute attention.

Includes supplementary material: sn.pub/extras

Klappentext
Recent major advances in our understanding of modulating protein functions has led to the development of new methods and algorithms to predict and decipher how amino acid sequences shape three-dimensional structures. Protein Design: Methods and Applications presents the most up-to-date protein design and engineering strategies so that readers can undertake their own projects with a maximum chance of success.
The authors present integrated computational approaches that require various degrees of computational complexity, and the major accomplishments that have been achieved in the design and structural characterization of helical peptides and proteins. Other topics of discussion include: design of structural elementary motifs, entire proteins, and interfaces of protein complexes, and of amyloidogenic polypeptides and amyloid inhibitors.
Authoritative and cutting-edge, Protein Design: Methods and Applications will be of major interest to protein scientists, biochemists, and all experimentalists selecting the strategy most adaptable for their design problem.

Inhalt
Design of Structural Elementary Motifs.- Structure and Stability of the ?-Helix.- De novo Design of Monomeric ?-Hairpin and ?-Sheet Peptides.- De novo Proteins From Binary-Patterned Combinatorial Libraries.- Non-Protein Amino Acids in the Design of Secondary Structure Scaffolds.- Design and Synthesis of ?-Peptides With Biological Activity.- Design Of Entire Proteins And Protein Complexes.- Design of Miniproteins by the Transfer of Active Sites Onto Small-Size Scaffolds.- Consensus Design as a Tool for Engineering Repeat Proteins.- Multiple Sequence Alignment as a Guideline for Protein Engineering Strategies.- Sequence Search Methods and Scoring Functions for the Design of Protein Structures.- Prediction of ProteinProtein Interaction Based on Structure.- Electrostatic Design of ProteinProtein Association Rates.- Design of Amyloidogenic Polypeptides and Amyloid Inhibitors.- Peptide Model Systems for Amyloid Fiber Formation Design Strategies and Validation Methods.- Protein Misfolding Disorders and Rational Design of Antimisfolding Agents.