PURIFICATION AND CHARACTERIZATION OF NOVEL BACTERIAL LIPASE

Over the past decade, chemical industries have come under increasing regulation by environmental protection agencies, highlighting the need for sustainable alternatives. In this context, biocatalysts have emerged as a driving force, as enzymes are highly specific, selective, and efficient. Among the many enzymes used in industrial processes, lipases stand out for their remarkable versatility and ability to catalyze a wide range of biotransformation reactions, even in organic solvents. This book presents a detailed scientific exploration into the purification and biochemical profiling of a novel lipase enzyme derived from Staphylococcus argenteus MG2 (MTCC 12820). This enzyme, purified using ammonium sulfate precipitation and advanced chromatographic techniques, demonstrates exceptional characteristics including high specific activity, broad pH and temperature stability, and remarkable resistance to surfactants and inhibitors. This book offers essential insights for researchers, academicians, and industry professionals in microbiology, enzymology, and biotechnology.

Autorentext

Dr. Mahima Golani, M.Sc., Ph.D. in Microbiology from UTD, DAVV, Indore (India), is Professor of Microbiology at PMB Gujarati Science College, Indore (MP) and research supervisor with expertise in enzyme technology. She has successfully completed project work and contributed to advancements in microbial and enzyme applications in biotechnology.