Quantum Dots: Applications in Biology

In Quantum Dots: Applications in Biology, Second Edition, expert researchers in the field detail consolidated approaches as well as new trends in the field. Organized into five parts, the first part comprises an introduction on Quantum Dots (QDs) as fluorescent probes in Life Sciences. While the second section covers important features about QDs´ preparative processes and characterizations for their successful application as fluorophores. The third part presents main aspects related to QDs methods applied to live cells and tissues. The fourth section focuses on QDs experiments in small animals and the fifth part demonstrates the versatility of QDs in a set of FRET applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Thorough and intuitive, In Quantum Dots: Applications in Biology, Second Edition aids scientists in continuing to study QDs by providing information about methods and protocols helping to expand their research.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Quantum Dots as Biophotonics Tools.- Photoligation Combined with Zwitterion-Modified Lipoic Acid Ligands Provides Compact and Biocompatible Quantum Dots.- Analysis of Quantum Dots and Their Conjugates by Capillary Electrophoresis with Detection of Laser-Induced Luminescence.- Advanced Procedure for Oriented Conjugation of Full-Size Antibodies with Quantum Dots.- Quantum Dots-Antibody Conjugates via Carbodiimide-Mediated Coupling for Cellular Imaging.- Measuring the Hydrodynamic Radius of Quantum Dots by Fluorescence Correlation Spectroscopy.- Quantum Dots Fluorescence Quantum Yield Measured by Thermal Lens Spectroscopy.- Semiquantitative Fluorescence Method for Bioconjugation Analysis.- Assembly, Characterization and Delivery of Quantum Dot Labeled Biotinylated Lipid Particles.- Oriented Conjugation of Single-Domain Antibodies and Quantum Dots.- Functional Integration of Quantum Dot Labeled Mesenchymal Stem Cells in a Cardiac Microenvironment.- In Vitro Approaches to Assessing the Toxicity of Quantum Dots.- Basics for the Preparation of Quantum Dots and their Interactions with Living Cells.- In Vivo Approaches to Assessing the Toxicity of Quantum Dots.- Quantum Dots for Imaging Neural Cells In Vitro and In VivoA Short-Term Inhalation Study Protocol Designed for Testing of Toxicity and Fate of Nanomaterials.- Quantitative Measurement of Proteolytic Rates with Quantum Dot-Peptide Substrate Conjugates and Förster Resonance Energy Transfer.- Solid-Phase Supports for the in situ Assembly of Quantum Dot-FRET Hybridization Assays in Microfluidics.