Rare-earth element biochemistry: Methanol dehydrogenases and lanthanide biology

Methods in Enzymology, Volume 650 continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Biophysical methods to study lanthanide-protein interactions, Genetically encoded sensors to study lanthanide biology, Spectrophotometric methods to determine the stability constants of lanthanide-macromolecule complexes, Lanthanide-based probes for amino acid modifications, In vitro selection and application of lanthanide-dependent DNAzymes, LRET biosensors for imaging protein interactions in living cells, Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor, EPR spectroscopy of lanthanides, Macromolecular crystallography for f-element complex characterization, and much more.

Autorentext
Joseph A. Cotruvo, Jr. is Assistant Professor, Louis Martarano Career Development Professor, Department of Chemistry
Pennsylvania State University, PA, USA

Inhalt

Discovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs * Tomoyuki Nakagawa

• Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor * Eric Schelter
• Activity assays of methanol dehydrogenases * Lena Daumann
• Expression, purification and properties of enzymes involved in lanthanide-dependent alcohol oxidation: XoxF4, XoxF5, ExaF/PedH, XoxG4 * Ludmila Chistoserdova
• Expression, purification and testing of lanthanide-dependent enzymes in Methylorubrum extorquens AM1 * Norma Cecilia Martinez-Gomez
• Heterologous expression, purification, and characterization of proteins involved in lanthanide utilization * Joseph A. Cotruvo Jr.
• Transposon mutagenesis for methylotrophic bacteria using Methylorubrum extorquens AM1 as a model system * Elizabeth Skovran
• Metals and Methylotrophy: Global Gene Expression Studies * Marina G. Kalyuzhnaya
• Mass spectrometry-based approaches to study lanthanides in the phyllosphere * Julia A. Vorholt
• Cultivation techniques to study lanthanide metal interactions in the haloalkaliphilic Type I methanotroph "Methylotuvimicrobium buryatense" 5GB1C *Joseph Douglas Groom Jr.*