Receptor Purification

The purpose of these volumes is to provide a reference work for the methods of purifying many of the receptors we know about. This becomes increasingly important as full-length recep tors are overexpressed in bacteria or in insect cell systems. A major problem for abundantly expressed proteins will be their purification. In addition to purification protocols, many other de tails can be found concerning an individual receptor that may not be available in standard texts or monographs. No book of this type is available as a compendium of purification procedures. Receptor Purification provides protocols for the purification of a wide variety of receptors. These include receptors that bind: neurotransmitters, polypeptide hormones, steroid hormones, and ligands for related members of the steroid supergene family and others including receptors involved in bacterial motion. The text of this information is substantial so as to require its publica tion in two volumes. Consequently, a division was made by grouping receptors depending upon the nature of their ligands. Thus, in volume 1 there are contributions on serotonin receptors, adrenergic receptors, the purification of GTP-binding proteins, opioid receptors, neurotensin receptor, luteinizing hormone re ceptor, human chorionic gonadotropin receptor, follicle stimulat ing hormone receptor, thyrotropin receptor, prolactin receptor, epidermal growth factor receptor, platelet derived growth factor receptor, colony stimulating factor receptor, insulinlike growth factor receptors, insulin receptor, fibronectin receptor, interferon receptor, and the cholecystokinin receptor.

Inhalt
Dexamethasone 21-Mesylate: Applications for Purification and Examination of Glucocorticoid Receptors.- Dexamethasone-Biotin Affinity Probes: Partial Purification of the Human Spleen Tumor Glucocorticoid Receptor.- Purification of the Unactivated/Nontransformed Glucocorticoid Receptor Complex from Rat Tissues.- Purification of the Glucocorticoid Receptor and Its Ligand-Binding Domains.- Glucocorticoid Receptor Purification.- Glucocorticoid Receptor Purification and Characterization Using Tissue Culture Cells.- The Glucocorticoid Receptor:Purification and Characterization.- Purification of the Rat Kidney Mineralocorticoid Receptor.- Purification of the Androgen Receptor.- Purification of the Estrogen Receptor.- Estrogen Receptor Purification.- Progesterone Receptor Purification.- Purification of the Nontransformed Avian Progesterone Receptor.- Purification and Characterization of Chicken Progesterone Receptor.- 1,25-Dihydroxyvitamin D Receptor Purification and Structural Determination.- Retinoic Acid-Binding Protein.- Receptor Purification of the Thyroid Hormone Receptor.- The Vasopressin V1 Receptor: Purification, Characterization and Analysis of VI Receptor Interaction(s) with GuanineNucleotide-Binding Proteins.- Purification of an Angiotensin II Receptor.- Purification of Prostaglandin E1/Prostacyclin Receptors of Human Blood Platelets.- The Asialoglycoprotein Receptor.- Periplasmic and Membrane Receptors of Bacterial Chemotaxis.- Index 413.