REGENERATION RESPONSE OF GLADIOLUS

Gladiolus Cultivar Friendship is highly susceptible
to soil born Fungus Fusarium oxysporum. Highly
resistant cell-lines were regenerated through
in-vitro selection at callus and cell suspension
level. Cell suspensions of all four Gladiolus
cultivars were found highly sensitive to fusaric
acid. Gradual increase in fusaric acid concentrations
to the cell-suspension cultures decreased cell growth
considerably. The cormlets of all in-vitro selected
cell lines of "Friendship" were inoculated with a
conidial suspension of the F. oxysporum. The four
selected cell lines showed the same response whether
or not inoculated with conidia of the F.
oxysporum. Plantlets of all of the selected cell
lines exhibited significant growth as compared with
control. RAPD markers were used to estimate
genetic diversity among the selected Fusarium
resistant cell-lines. Marker data was generated
through three successive generations. The number of
fragments per primer ranged from 4 to 10. The total
size of the amplified products varied from 200bp to
1800bp. All the primers were found to be polymorphic
and produced upto 62% polymorphism.

Autorentext

DR. IDREES AHMAD NASIR, Assistant Professor, CEMB,PunjabUniversity, Lahore, Pakistan has 20 years Teaching and ResearchExperience in Plant Molecular Biology. He has Developed FusariumResistant Gladiolus, Hybrids of Tomato, Corn, Bt-Corn. He alsodeveloped virus free/resistant Potatoes, Sugarcane and SyntheticSeeds of Carrot and Cucumber.

Klappentext

Gladiolus Cultivar Friendship is highly susceptibleto soil born Fungus Fusarium oxysporum. Highlyresistant cell-lines were regenerated throughin-vitro selection at callus and cell suspensionlevel. Cell suspensions of all four Gladioluscultivars were found highly sensitive to fusaricacid. Gradual increase in fusaric acid concentrationsto the cell-suspension cultures decreased cell growthconsiderably. The cormlets of all in-vitro selectedcell lines of "Friendship" were inoculated with aconidial suspension of the F. oxysporum. The fourselected cell lines showed the same response whetheror not inoculated with conidia of the F.oxysporum. Plantlets of all of the selected celllines exhibited significant growth as compared withcontrol. RAPD markers were used to estimategenetic diversity among the selected Fusariumresistant cell-lines. Marker data was generatedthrough three successive generations. The number offragments per primer ranged from 4 to 10. The totalsize of the amplified products varied from 200bp to1800bp. All the primers were found to be polymorphicand produced upto 62% polymorphism.