Rhinoviruses

Rhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as being more recently recognized as the major viral cause of asthma and chronic obstructive pulmonary disease (COPD) exacerbations. The huge advancements in technical applications of biophysics and improvements in high-end imaging techniques have also had implications for HRV research among other infectious diseases. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Rhinoviruses: Methods and Protocols seeks to serve both professionals and novices with the latest technical advances in HRV research to ultimately enable the development of therapeutic approaches to combat HRV, and most importantly, its pathogenic effects in asthma exacerbation.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Classification and Evolution of Human Rhinoviruses.- Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples.- Molecular Identification and Quantification of Human Rhinoviruses in Respiratory Samples.- Molecular Genotyping of Human Rhinovirus by PCR and Sanger Sequencing.- Growth of Human Rhinovirus in H1-Hela Cell Suspension Culture and Purification of Virions.- Propagation of Rhinovirus C Strains in Differentiated Epithelial Cells of Human Airway at Air-Liquid Interface.- Infectivity Assays of Human Rhinovirus-A and B Serotypes.- Application of FCS in Studies of Rhinovirus Receptor Binding and Uncoating.- Capillary Electrophoresis, Gas Phase Electrophoretic Mobility Molecular Analysis, and Electron Microscopy - Effective Tools for Quality Assessment and Basic Rhinovirus Research.- Proteases of Human Rhinovirus Role in Infection.- A Protocol to Express and Isolate HRV16 3C Protease for Use in In vitro Protease Assays.- Reverse Genetics System for Studying Human Rhinovirus Infections.- Reverse Genetic Engineering of the Human Rhinovirus Serotype 16 Genome to Introduce an Antibody-Detectable Tag.- Mouse Models of Rhinovirus Infection and Airways Disease.